卵黄状黄斑病蛋白2抗体elisa实验

卵黄状黄斑病蛋白2抗体英文名称  Anti-Bestrophin 2 

中文名称  卵黄状黄斑病蛋白2抗体 

别    名  BEST 2; Best disease Bestrophin; BEST2; Bestrophin2; FLJ20132; Vitelliform macular dystrophy 2 homolog; Vitelliform macular dystrophy 2 like 1; Vitelliform macular dystrophy 2 like protein 1; Vitelliform macular dystrophy; VMD2; VMD2 like gene 1; VMD2L1; BEST2_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg 

卵黄状黄斑病蛋白2抗体抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep   

产品类型  一抗    

研究领域  肿瘤 神经生物学 信号转导 通道蛋白  

蛋白分子量  predicted molecular weight: 57kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human Bestrophin 2 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The Bestrophins are a newly described family of anion channels unrelated in primary sequence to any previously characterized channel proteins. Bestrophins were originally defined as a family of over 20 related sequences of the C. elegans. The first mammalian Bestrophin was identified as the vitelliform macular dystrophy (VMD), 1 also known as Best disease. Three more members of the bestrophin family members were cloned and indentified recently, Bestrophin 2, 3 and 4. RT PCR analyses revealed tissue restricted expression of the three genes with both Bestrophin 1 and Bestrophin 2 are abundantly transcribed in colon. Functionally the bestrophines oligomerise to form tetramers and pentamers in order to act as calcium sensitive chloride channels. It has been shown that Bestrophin interacts with beta catalytic subunit of protein phosphatase 2A (PP2Ac). Such protein protein interaction between Bestrophin and PP2Ac and the 卵黄状黄斑病蛋白2抗体structural subunit of PP2A, PR65, was confirmed by reciprocal immunoprecipitation. The interaction between PP2Ac and the Bestrophin takes place near the carboxy terminal end of the protein.

Function : Forms calcium-sensitive chloride channels. Permeable to bicarbonate. 

Subcellular Location : Cell membrane; Multi-pass membrane protein. 

Tissue Specificity : Mainly confined to the retinal pigment epithelium and colon.

Similarity : Belongs to the bestrophin family.

Database links : UniProtKB/Swiss-Prot: Q8NFU1.1