溴脱氧尿苷抗体elisa实验

参考价:面议
供货周期: 现货
品牌: GenWay
规格: 0.1ml/100μg 0.2ml/200μg
货号: BJ-3860
CAS号:
上海邦景实业有限公司
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产品详情

溴脱氧尿苷抗体英文名称  Anti-BrdU 

中文名称  溴脱氧尿苷抗体 

别    名  Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg  1ml/1mg 

抗体来源  Rabbit  

溴脱氧尿苷抗体克隆类型  polyclonal 

交叉反应  BrdU  

产品类型  一抗  药物与化合物抗体   

研究领域  细胞生物  

蛋白分子量  predicted molecular weight: 20/48kDa 

性    状  Lyophilized or Liquid 

免 疫 原  BrdU conjugated to KLH 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

(石蜡切片需做抗原修复) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine 溴脱氧尿苷抗体analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.

BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis 

Subcellular Location : Nuclear.


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