丁酰胆碱酯酶单克隆抗体elisa实验

丁酰胆碱酯酶单克隆抗体英文名称  Anti-BCHE(1E8) 

中文名称  丁酰胆碱酯酶单克隆抗体 

别    名  Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase. CHLE_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  1ml/1mg 

丁酰胆碱酯酶单克隆抗体抗体来源  Mouse  

克隆类型  polyclonal 

克 隆 号  1E8 

交叉反应  Human,   

产品类型  一抗    

研究领域  心血管  

蛋白分子量  predicted molecular weight: 66kDa 

性    状  Lyophilized or Liquid 

免 疫 原  Full length native protein purified from human 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008].

Function : Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Subunit : Homotetramer; disulfide-linked. Dimer of dimers.

Subcellular Location : Secreted.

Tissue Specificity : Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

DISEASE : Butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]: Metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium 丁酰胆碱酯酶单克隆抗体and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the type-B carboxylesterase/lipase family.

Database links : UniProtKB/Swiss-Prot: P06276.1 

血清丁酰胆碱酯酶是由四个亚基组成的糖蛋白，分子量28万～35万。作为一类糖蛋白，主要存在于脑、肝、血及胆碱能神经末稍的突触间隙中，主要由肝脏合成，并存在于血清中，生理作用不明，称为假性胆碱酯酶或丁酰胆碱酯酶[1]Lockridge等采用Edman降解法测定了人血清BChE完整的氨基酸序列，共含有574个氨基酸残基。BChE氨基酸序列与其他酯酶家族蛋白有很高的同源性。以电鳐AChE的三维结构为模板对人BChE的三维结构进行计算机模拟结果表明，BChE活性中心，囊袋的底部为Ser198，其两侧相距一定距离分别是酰基结合部位和胆碱结合部位。研究发现人BChE的氨基酸序列上有Zn2+离子结合结构。