多聚合苷酸激酶3磷酸化酶抗体elisa实验

多聚合苷酸激酶3磷酸化酶抗体英文名称  Anti-PNK1/PNKP 

中文名称  多聚合苷酸激酶3磷酸化酶抗体 

别    名  Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; PNK 1; PNK; PNK1; PNKP; Polynucleotide 5' hydroxyl kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; PNKP_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg  

多聚合苷酸激酶3磷酸化酶抗体抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Cow, Sheep  

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 57kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human PNK1/PNKP 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Mammalian PNK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. It plays an important function in DNA repair following ionizing radiation or oxidative damage. PNK has been reported to participate in the repair of DNA-double strand breaks via PARP-1-dependent nonhomologous end-joining.

Function : Plays a key role in the repair of DNA damage,functioning as part of both the non-homologous end-joining (NHEJ)and base excision repair (BER) pathways. Through its two catalyticactivities, PNK ensures that DNA termini are compatible withextension and ligation by either removing 3'-phosphates from, or byphosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNAbackbone.

Subunit : Monomer (By similarity). 

Subcellular Location : Nucleus. 

Tissue Specificity : Expressed in many tissues with highestexpression in spleen and testis, and lowest expression in smallintestine (PubMed:10446192). Expressed in higher amount inpancreas, heart and kidney and at lower levels in brain, lung andliver (PubMed:10446193).

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. 

DISEASE : Defects in PNKP are the cause of epilepticencephalopathy, early infantile, type 10 (EIEE10) [MIM:613402]. Adisease characterized by microcephaly, infantile-onset seizures,severe intellectual disability and 多聚合苷酸激酶3磷酸化酶抗体delayed motor milestones withabsent speech or only achieving a few words. Most patients alsohave behavioral problems with hyperactivity. Microcephaly isprogressive and without neuronal migration or structuralabnormalities, consistent with primary microcephaly.

Similarity : In the N-terminal section; belongs to the DNA 3'phosphatase family.

Contains 1 FHA domain. 

Database links : UniProtKB/Swiss-Prot: Q96T60.1