| 型号： | Vena8 Endothelial+™ biochip |
| 产地： | 爱尔兰 |
| 品牌： | |
| 评分： |
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VENA8 ENDOTHELIAL+微流体芯片的主要特点：
用于细胞滚动、粘附分子研究或细胞间相互作用研究
适用于各种细胞悬浮液(原代和细胞株)包括T细胞、单核细胞、外周血单个核细胞、中性粒细胞、嗜酸性粒细胞、血小板和全血(肝素化)。
Vena8荧光+ TM和 Vena8TM：可以用标准移液器将一系列的粘附因子包覆毛细管外壁。不同的黏附分子包括VCAM(血管细胞粘附因子),ICAM(细胞间粘附因子),MAdCAM，纤维连接蛋白，vWF(人血管性血友病因子/瑞斯托霉素辅因子)、胶原蛋白、纤维蛋白原等。
Vena8内皮细胞+ TM和VenaECTM：容易种植和培养各种内皮细胞(层)包括HUVEC(人脐静脉血管内皮细胞)，HMVEC(人微血管内皮细胞)，HCAECs(人冠状动脉内皮细胞)等等。
生物芯片具有良好的光学特性，可在显微镜下进行清晰的观察和进一步的研究。
剪切应力范围：0.05-20 dyne/ cm2，可以通过MirusTM Nanopump精确控制。
剪切应力的大小及连续变化的参数可以设置。
可在流动状态下实时成像。
The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
Features:
Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.
Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.
Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
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