型号: | COPAS微模式生物自动分选系统,COPAS Platform |
产地: | 美国 |
品牌: | |
评分: |
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微型模式动物、模式植物、大体积细胞等在生命科学研究中有着非常广泛的应用,但是由于这些对象体积太大,普通的流式细胞仪的粒径尺寸一般在几十微米,难以对秀丽线虫、斑马鱼这样的大粒径模式生物进行分析和分选;手工镜下分选非常耗时耗力、效率低下、准确性难以保证。COPAS高通量分选系统是一款特殊设计的分选型流式细胞仪,流通室径向尺寸可达2000微米,可以对秀丽线虫、蚕卵、斑马鱼卵及幼鱼、果蝇等模式生物进行高通量、自动化的分析和分选。
COPAS高通量分选系统能够很大程度上提高了分选效率,完成大规模的样品分选工作,实现高通量分选。还可对多种数据进行分析,可以开展用传统实验手段难以开展的课题实验。
COPAS高通量分选系统在国际上有数百家实验室的成功应用案例,在很多知名期刊也有很多引用文献。该设备的应用,能够帮助实验室在很大程度上提高实验效率、拓展科研方法、缩短科研产出时间。
产品型号:COPAS FP
主要特点:
可分选粒径20-1500um大颗粒物、细胞群、模式生物
全自动、快速、精确分选和分析
提供分选颗粒数字化定量数据,可用于用户对目标群体的统计分析
无菌环境及温和气流分选方式,能够有效保持分选样品的洁净和存活率
主要功能:
? 高通量分选能够很大程度上提高分选效率,同时可以开展用传统试验方法难以开展的实验
? 设备通过检测样品的多种光学信号,依据检测到的信号进行自动化分选,排除了人为主观因素:分选更客观,更准确
? 设备可以把生物样本自动分选到24,48,96或384孔板中,也可以自定义,实现分选的自动化
? COPAS高通量分选系统是在常规分选型流式细胞仪基础上研发和改进而成,流动室内压力小,采用温和的气流分选方式,可以确保生物体的活性和完整性,对后续培养、分析甚至是再次分选不会产生负面影响
? 系统自动存储每个模式生物、微粒的光学数据,可用于进一步的统计和分析
? 可根据需要,选配专业化分析软件:能够在样本径向长度方向上进行多达8000个断层的光学监测,给出细胞-器官水平的光学参数信息,包括尺寸、光密度和荧光强度(例如斑马鱼心脏的神经元上的荧光信号),并以峰值曲线图的形式导出,可提供峰的个数、峰宽、峰高和相对位置等各种参数
? 仪器操作简单,一个人便可完成大规模的样品分选工作,实现高通量分选
主要应用:
种群差异分析(Population differences analysis)
·成长阶段分选
·药物筛选、化学诱导
荧光表达筛选(Fluorescence expression sorting)
·单荧光筛选
·多荧光筛选
基因表达定位分析(Gene expression localization analysis)
·荧光峰值筛选
·样品亚群分析
荧光定量分析(Fluorescent quantitative analysis)
工作原理及主要配置:
工作原理
通过获取多种光学参数径向尺寸、光密度、三色荧光强度对微粒进行分选
分选方式
采用温和的气流分选方式,能够很大程度上保障分选后生物微粒的生物活性和完整性(可对同一个样品多次分选)
光学部件布局
COPAS高通量分选系统的光学原件布局俯视图
流动室
每个胚胎、微粒或斑马鱼被鞘液包裹,依次通过流动室,在流经过程中测量出各种分选参数;
激光器
配备488nm激光器,用于测量物体的轴向长度和光学密度,还可以用来激发荧光
根据研究需要,可调整激光器的配置,有多种型号激光器可选:375nm,405nm,488nm,561nm或640nm
分选参数
通过检测和分析生物微粒的多种参数,进行分选
飞行时间(TOF)、透光度(EXT)、荧光强度(FLU) ,同时检测多达三种颜色的荧光
基础分析软件
分选参数设置、分选控制与数据分析
Profile高阶分析软件
具备多参数峰值特性分选功能
生物微粒通过流动室的过程中,系统对生物微粒轴向方向的光密度和荧光强度进行量化;
下图是使用Profile软件,Profiling GFP-expressing seam cells in nematodes(牛津大学伍拉德实验室)
LP Sampler自动进样器
能够实现对多孔板样品的自动化加样;
完整的高通量分选平台
COPAS斑马鱼高通量分选系统能可配合LP Sampler组成一套更加完整的高通量分选平台;
能够实现从多孔板进样到多孔板分选的全自动化操作;
What’s new with COPAS?
In the most recent update of the COPAS platform we have upgraded the system electronics and this moved COPAS to the newer, friendlier, more powerful FlowPilot software suite. This change is denoted by the name COPAS FlowPilot, abbreviated as COPAS FP. In this new version the model names COPAS BIOSORT, SELECT, PLUS and XL have been replaced by the more meaningful COPAS FP-250, FP-500, FP-1000 and FP-2000.
The COPAS FP platform is designed for the measurement of objects ranging in size from 20–1500 microns (diameter). Each of the four COPAS FP models features a fixed, specially engineered fluidic path, flow cell and optics optimized for a specific subset of that size range to provide maximum speed, accuracy, resolution and throughput, depending on the application. Instruments can dispense objects either into bulk receptacles or multiwell plates.
A 488 nm solid state laser is used to measure both the size and optical density of the objects, as well as for fluorescence excitation. Each COPAS FP system can be equipped with up to three lasers.
The real-time analysis of these measured parameters is used to make sort decisions and only those objects meeting the user-selected sort criteria are gently dispensed into multiwell plates or stationary receptacles. Those objects not meeting the sort criteria are diverted by a puff of air to a sample recovery container, where they may be recaptured, unharmed and still viable.
Instruments are equipped with an X-Y stage to permit the dispensing of objects into micro-wells, including 24-, 48-, 96-, and 384-well plate formats. Using the FlowPilot™ software, users may custom design templates or use default templates.
Factory trained personnel will install your new COPAS FP system and train you at your site in the operation and routine maintenance of the system and accessories.
All systems are supplied with a Windows® workstation running FlowPilot™ software for operation and analysis.
Choosing a COPAS FP Model
Each COPAS FP system features a specially engineered fluidic path and flow cell optimized for a specific object size range to achieve the highest accuracy and sensitivity possible. The table shown at Choosing the Right Platform and Model summarizes the largest object size recommended for each COPAS FP model and also provides general system recommendations based on some sample applications. The applications listed are not exhaustive, but simply meant to provide a general overview of system capabilities. For a more detailed discussion of your particular application, including those applications that are not listed here, please contact our applications scientists directly at yuyan0317@126.com.
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