氢离子转运ATP合成酶6G抗体

氢离子转运ATP合成酶6G抗体

产品编号        LM10930R    

英文名称    ATP6V1G2    

中文名称    氢离子转运ATP合成酶6G抗体    

别    名    ATP6G; ATP6G2; ATPase H+ transporting lysosomal (vacuolar proton pump) subunit G; ATPase H+ transporting lysosomal 13kDa V1 subunit G2; H(+) transporting two sector ATPase subunit G2; NG 38; NG38; V ATPase 13 kDa subunit 2; V ATPase G subunit 2; V ATPase subunit G 2; Vacuolar ATP synthase subunit G 2; Vacuolar proton pump G subunit 2; Vacuolar proton pump subunit G 2; VMA 10; VMA10.      

研究领域    肿瘤  细胞生物  免疫学  信号转导      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    14kDa    

细胞定位    细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ATP6V1G2:51-118/118     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene. [provided by RefSeq, Feb 2011]    

抗原抗体结合特点

 1）特异性：抗原分子只能与由它刺激所产生的抗体结合而起反应的专一性能；

 2）可逆性：是指Ag与相应Ab结合成IC后，在一定条件下可解离为游离抗原与抗体。

 3）阶段性：

 一阶段：特异性结合阶段 （①反应速度快，几秒钟或几分钟即可完成 ②不出现肉眼可见反应）；

 第二阶段：可见阶段（表现为凝集、沉淀、补体结合等反应 ①反应进行慢，需要几分钟、几十分钟或更长②受电解质、温度、酸碱度等多种因素影响）。

4）比例性：（最适比或等价点）是指抗原与抗体发生可见反应需遵循一定的量比关系，只有当二者浓度比例适当时，才出现可见反应。

抗原抗体反应影响因素：

（一）反应物自身的因素

5） 抗体：不同来源的抗体,反应性各有差异,抗体的浓度、特异性和亲和力都影响抗体抗原反应,为提高试验的可靠性,应选择高特异性、高亲和力的抗体作诊断试剂.等价带的宽窄也影响抗原抗体复合物的形成,单克隆抗体不适用于沉淀反应.

6）  抗原：抗原的理化性状、分子量、抗原决定簇的种类及数目均可影响反应结果.颗粒性抗原出现凝集反应,可溶性抗原出现沉淀反应,单价抗原与相应抗体结合不出现沉淀现象.

（二）反应环境条件

7） 酸碱度：抗原抗体反应必须在合适的pH环境中进行.蛋白质具有两性电离性质,因此每种蛋白质都有固定的等电点.抗原抗体反应一般在pH6～9进行,有补体参与的反应pH为7.7.4,pH过高或过低都将影响抗原与抗体反应.

8）  温度：在一定范围内,温度升高可加速分子运动,抗原与抗体碰撞机会增多,使反应加速.一般为15℃～40℃,常用的抗原抗体反应温度为37℃,温度如高于56℃,可导致已结合的抗原抗体再解离,甚至变性或破坏.每种试验都有其独特的最适反应温度要求.此外,适当振荡也可促进抗原抗体分子的接触,加速反应.

9）  电解质：抗原与抗体发生特异性结合后,虽由亲水胶体变为疏水胶体,若溶液中无电解质参加,仍不出现可见反应.为了促成沉淀物或凝集物的形成,常用0.85%NaCl或各种缓冲液作为抗原及抗体的稀释液.