供货周期: | 现货 |
品牌: | LMAI Bio |
规格: | 50ul/100ul/200ul |
货号: | LM13673R |
CAS号: |
Src家族相关磷酸化蛋白2抗体
产品编号 LM13673R
英文名称 SCAP2
中文名称 Src家族相关磷酸化蛋白2抗体
别 名 Fyn associated phosphoprotein SKAP55 homologue; MGC10411; MGC33; PRAP; Pyk2/RAFTK associated protein; Pyk2/RAFTK-associated protein; RA70; Retinoic acid-induced protein 70; SAPS; SKAP-55HOM; SKAP-HOM; skap2; SKAP2_HUMAN; SKAP55 homolog; SKAP55R; Src associated adaptor protein; Src family associated phosphoprotein 2; Src family-associated phosphoprotein 2; src kinase associated phosphoprotein of 55 related protein; Src kinase-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domains.
研究领域 细胞生物 信号转导 激酶和磷酸酶 淋巴细胞
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Horse, Rabbit, Sheep,
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 41kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SCAP2:1-100/359
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.
抗原与抗体的关系
①抗体和相应的抗原结合后,可以促进白细胞的吞噬作用而将抗原消除,使抗原失去致病作用。
②一种抗体只能抵抗一种抗原,而且一种抗体只能受相应的抗原刺激后才能形成。
免疫的功能:
免疫具有三方面的功能:
①防御功能:抵抗抗原的侵入、防止疾病的产生。
②自我稳定:清除体内衰老、死亡和损伤的细胞。
③免疫监视:监视、识别和清除体内产生的异常细胞。
特别提醒:过敏反应:当人体抵抗抗原侵入的功能过强时,在过敏原(引起过敏反应的物质,如某些食物、药物)的刺激下,人体就会发生过敏反应。
1.抗体名称容易出错
抗体一般以抗原来命名,名称核对时最简单也最容易犯错,有些蛋白质有不同亚基(HIF-1α、HIF-1β);有些蛋白质有不同磷酸化状态,例如EGFR和p-EGFR的区别,等等。
除了蛋白质的名称外,有其他后缀。
2.多抗和单抗不同
单抗上面介绍过了,而多抗(polyclonal antibodies)一般用兔子、山羊等大型动物,因为多抗是直接从血液中纯化提取出来的,而不像单抗是用细胞培养出来的。
单克隆抗体的优点是产量持续稳定、特异性较高、但灵敏度不如多抗。如果对抗体的特异性要求高,用量较大或需要长期使用一致的抗体,制备的抗体应用要求多(WB/IP/IF/ICC等),可以选择单克隆抗体。
多抗的特异性较差,存在批间差(因为兔子的血被放光挂掉了,而山羊可以常年养着,放点血出来,在养几天再放血,好残忍),易造成背景,例如在WB中有杂带,在IHC中背景较深等等。但由于多抗识别多个抗原表位,对于丰度偏低的蛋白也更容易检出。
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