供货周期: | 现货 |
品牌: | LMAI Bio |
规格: | 50ul/100ul/200ul |
货号: | LM19547R |
CAS号: |
蛋白磷酸酶2调节亚基2C抗体
产品编号 LM19547R
英文名称 PPP2R2C
中文名称 蛋白磷酸酶2调节亚基2C抗体
别 名 2ABG_HUMAN; B55 gamma; Gamma isoform of regulatory subunit B55 protein phosphatase 2; IMYPNO 1; IMYPNO; IMYPNO1; MGC33570; OTTHUMP00000115505; Phosphoprotein phosphatase 2A BR gamma regulatory chain; PP2A subunit B B gamma isoform; PP2A subunit B B55 gamma isoform; PP2A subunit B isoform B55-gamma; PP2A subunit B isoform gamma; PP2A subunit B isoform PR55-gamma; PP2A subunit B isoform R2-gamma; PP2A subunit B PR55 gamma isoform; PP2A subunit B R2 gamma isoform; PPP2R2C; PPP2R2C protein; PR 52; PR 55G; PR52; PR55G; Protein phosphatase 2 (formerly 2A) regulatory subunit B gamma isoform; Protein phosphatase 2 regulatory subunit B gamma; Protein phosphatase 2 regulatory subunit B gamma isoform; Protein phosphatase 2A1 B gamma subunit; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform.
研究领域 细胞生物 信号转导 激酶和磷酸酶
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PPP2R2C:1-100/447
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
抗原与抗体的关系
①抗体和相应的抗原结合后,可以促进白细胞的吞噬作用而将抗原消除,使抗原失去致病作用。
②一种抗体只能抵抗一种抗原,而且一种抗体只能受相应的抗原刺激后才能形成。
免疫的功能:
免疫具有三方面的功能:
①防御功能:抵抗抗原的侵入、防止疾病的产生。
②自我稳定:清除体内衰老、死亡和损伤的细胞。
③免疫监视:监视、识别和清除体内产生的异常细胞。
特别提醒:过敏反应:当人体抵抗抗原侵入的功能过强时,在过敏原(引起过敏反应的物质,如某些食物、药物)的刺激下,人体就会发生过敏反应。
1.抗体名称容易出错
抗体一般以抗原来命名,名称核对时最简单也最容易犯错,有些蛋白质有不同亚基(HIF-1α、HIF-1β);有些蛋白质有不同磷酸化状态,例如EGFR和p-EGFR的区别,等等。
除了蛋白质的名称外,有其他后缀。
2.多抗和单抗不同
单抗上面介绍过了,而多抗(polyclonal antibodies)一般用兔子、山羊等大型动物,因为多抗是直接从血液中纯化提取出来的,而不像单抗是用细胞培养出来的。
单克隆抗体的优点是产量持续稳定、特异性较高、但灵敏度不如多抗。如果对抗体的特异性要求高,用量较大或需要长期使用一致的抗体,制备的抗体应用要求多(WB/IP/IF/ICC等),可以选择单克隆抗体。
多抗的特异性较差,存在批间差(因为兔子的血被放光挂掉了,而山羊可以常年养着,放点血出来,在养几天再放血,好残忍),易造成背景,例如在WB中有杂带,在IHC中背景较深等等。但由于多抗识别多个抗原表位,对于丰度偏低的蛋白也更容易检出。
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