磷酸化表皮生长因子受体抗体

磷酸化表皮生长因子受体抗体

英文名称    Phospho-EGFR (Ser1045)    

中文名称    磷酸化表皮生长因子受体抗体    

别    名    EGFR(Phospho-Ser1045); EGFR (phospho Ser1045); EGFR (phospho S1045); p-EGFR (Ser1045); p-EGFR (S1045); EGFR; Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Avian erythroblastic leukemia viral (verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  神经生物学  信号转导  生长因子和激素  细胞膜受体      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Horse,     

产品应用    WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /test IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    130kDa    

细胞定位    细胞核 细胞浆 细胞膜 分泌型蛋白     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser1045:NN(p-S)TV     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.    

细胞膜受体（Membrane Receptors)
    EGFR-血管内皮生长因子受体EGFR是一类分子量为170kDa的糖蛋白，在生长的细胞包括中瘤细胞中跨越细胞质膜，表现有蛋白激酶活性。
    EGFR是一种细胞膜受体激酶，对血管内皮生长因子有高度的亲和性，主要功能是参与血管内皮细胞生长和血管生成的调控,主要用于各种恶性肿瘤的研究.与其配体表皮生长因子或尿抑胃素结合后则被激活，从而启动DNA及蛋白质的合成。再不进行有丝分裂的细胞中并不存在，但在胃中例外。
    大量研究报道显示，EGFR高表达的肿瘤生存降低、转移风险增高、预后不良。在很多肿瘤中都存在着EGFR表达或过度表达。这些疾病包括：结直肠癌（CRC）、头颈部鳞状细胞癌（SCCHN）、乳腺癌、卵巢癌、宫颈癌、食道癌、胰腺癌、膀胱癌、前列腺癌和非小细胞肺癌等。研究表明，EGFR表达的肿瘤恶性程度增高、侵袭性强，这类肿瘤患者往往生存降低、转移风险增高、预后不良。