磷酸化粘着斑激酶抗体

磷酸化粘着斑激酶抗体

英文名称    Phospho-FAK (Tyr861)    

中文名称    磷酸化粘着斑激酶抗体    

别    名    FAK (phospho Y861); p-FAK (phospho Tyr861); FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  神经生物学  信号转导  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Cow, Horse,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    116kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr861:HI(p-Y)Q     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.    

FAK是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化,FAK本身是胱冬肽酶(caspase)的底物。作为信号分子的FAK参与抑制细胞凋亡并直接参与细胞多种功能的调节。
1.FAK 局部粘着斑激酶，是一种酪氨酸激酶;肿瘤细胞的侵袭性生长是一个多步骤的复杂过程，有多种生物化学因子参与其中,局部粘着斑激酶（focal adhesion kinase, FAK）介导的信号转导系统就是其中最为重要的细胞信号转导途径之一。肿瘤细胞必须黏附于细胞外基质，通过促进依赖于PTK激酶活性的细胞外基质信号转导，进而影响细胞的黏附、运动与迁移。 
2.粘着斑激酶(focal adhesion kinase,FAK)是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化；为信号分子的FAK,还与细胞内其他信号转导通路存在串话(crosstalk),直接参与了细胞多种功能的调节。 
3.尽管FAK的确切功能尚不清楚,但若干实验均提示FAK可能有两个作用,一是在细胞铺展和移动时,FAK参与粘着斑形成和调节；二是FAK参与信号转导过程,以告知细胞核其细胞已锚定了。近年有关FAK在细胞凋亡中的作用也业已肯定。