白细胞共同抗原抗体

白细胞共同抗原抗体

英文名称    CD45    

中文名称    白细胞共同抗原抗体    

别    名    B220; CD 45; CD45; cd45 antigen; ec3.1.3.48; GP180; GP 180; L CA; LCA; L-CA; Leukocyte common antigen; LY5; Protein tyrosine phosphatase receptor type C; Protein tyrosine phosphatase receptor type c polypeptide; protein tyrosine phosphatase, receptor type, C; Receptor-type tyrosine-protein phosphatase C; PTPRC; PTPRC_HUMAN; SCID due to PTPRC deficiency; T200; T200 glycoprotein; T200 leukocyte common antigen; Human homolog of severe combined immunodeficiency due to PTPRC deficiency.      

研究领域    肿瘤  细胞生物  免疫学  神经生物学  信号转导  干细胞  转录调节因子  细胞表面分子  糖蛋白  细胞类型标志物  自然杀伤细胞  淋巴细胞  t-淋巴细胞  b-淋巴细胞      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Mouse,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    141kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from mouse CD45/B220:401-500/1343 <Extracellular>    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008].    

CD45在活化信号转导中起到调节作用 在确定CD45为一种PTPase之前就已证实了CD45参于细胞的活化和生长调节。

抗CD45抗体可以抑制PHA或CD3交联所介导的T细胞增殖，还可抑制NK或细胞毒性T细胞对靶细胞的杀伤，抑制经CD2、CD3以及CD8膜分子介导的信号转导作用。
白细胞共同抗原是五种或更多的高分子量糖蛋白组成的蛋白家族，主要位于白细胞表面，包括T、B淋巴细胞、多形核白细胞、单核细胞等，而在红细胞、血小板及非造血系统中不表达。因此是区分淋巴瘤/白血病和非造血组织肿瘤（如未分化小细胞癌、小圆细胞肉瘤）的特异性标记物。该抗体主要用于淋巴瘤和未分化小细胞癌的鉴别诊断。