磷酸化细胞周期检查控制蛋白质抗体

磷酸化细胞周期检查控制蛋白质抗体

英文名称    phospho-RAD9 (Tyr28)    

中文名称    磷酸化细胞周期检查控制蛋白质抗体    

别    名    RAD9A(phospho Y28); Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A; RAD9A_HUMAN.      

产品类型    磷酸化抗体     

研究领域    免疫学  染色质和核信号  信号转导  细胞凋亡  细胞周期蛋白  转录调节因子      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    43kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human RAD9 around the phosphorylation site of Tyr28:EL(p-Y)LE     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq].    

Rad9由391个AA构成，Rad9有很多重要生物学功能，如：可调控DNA损伤反应、调节细胞的周期、对损伤的DNA进行修复、诱导细胞凋亡、调控部分基因的转录、具有3’端到5’端核酸外切酵素(exonuclease)的活性、促进核糖核酸的生合成以及参与胚胎发育过程。曾有报道指出Rad9在调节细胞周期、诱导细胞凋亡及维持整个基因体的完整性等方面的生物功能都和p53极为相似，因此Rad9及p53这两种蛋白在功能上可能具有相互协调作用。