3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)

3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)

英文名称    GAPDH-Loading Control    

中文名称    3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)    

别    名    38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.      

研究领域    肿瘤  细胞生物  免疫学  神经生物学  新陈代谢      

抗体来源    Mouse    

克隆类型    Monoclonal    

克 隆 号    4F8    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit, Sheep, Hamster, Monkey,     

产品应用    WB=1:5000-10000  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    38kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from human GAPDH:     

亚    型    IgG    

纯化方法    affinity purified by Protein G    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.    

GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。
GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。
甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。
GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.