供货周期: | 一周 |
品牌: | 爱必信 |
规格: | 10ug |
货号: | abs00894 |
CAS号: |
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产品描述: 产品名称:Recombinant Human IGF-1, 15N 氨基酸序列: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA 参考文献: 1. Skottner A, Fryklund L, Hansson HA. 1986. Acta Paediatr Scand Suppl, 325: 107-11.<br/>2. Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.<br/>3. Palmade F, Sechoy-Chambon O, Coquelet C, et al. 1994. Curr Eye Res, 13: 531-7.<br/>4. Tennagels N, Hube-Magg C, Wirth A, et al. 1999. Biochem Biophys Res Commun, 260: 724-8.<br/>5. Laron Z. 2004. Novartis Found Symp, 262: 56-77; discussion -83, 265-8.<br/>6. Shiratsuchi I, Akagi Y, Kawahara A, et al. 2011. Anticancer Res, 31: 2541-5. Accession: <a href="http://www.uniprot.org/uniprot/P05019" target="_blank">P05019</a> Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/3479" target="_blank">3479</a> 种属: Human 纯度: > 97 % by SDS-PAGE and HPLC analyses. 来源: Escherichia coli. 内毒素水平: Less than 1 EU/μg of Recombinant HumanIGF-1, 15N as determined by LAL method. 活性: Fully biologically active when compared to standard. The ED<sub>50</sub> as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10<sup>5</sup> IU/mg. 溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2. 储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
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