供货周期: | 一周 |
品牌: | 爱必信 |
规格: | 5ug |
货号: | abs00943 |
CAS号: |
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产品描述: 产品名称:Recombinant Human Noggin 氨基酸序列: MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC 参考文献: 1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60.<br/>2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71.<br/>3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6.<br/>4. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56.<br/>5. Secondini C, Wetterwald A, Schwaninger R, et al. 2011. PLoS One, 6: e16078. Accession: <a href="http://www.uniprot.org/uniprot/Q13253" target="_blank">Q13253</a> Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/9241" target="_blank">9241</a> 种属: Human 纯度: > 95 % by SDS-PAGE and HPLC analyses. 来源: Escherichia coli. 内毒素水平: Less than 1 EU/μg of Recombinant HumanNoggin as determined by LAL method. 活性: Fully biologically active when compared to standard. The ED<sub>50</sub> as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 10<sup>5</sup> IU/mg in the presence of 5 ng/ml Recombinant HumanBMP-4. 溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 形态: Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. 储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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