供货周期: | 一周 |
品牌: | 爱必信 |
规格: | 5ug |
货号: | abs01012 |
CAS号: |
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产品描述: 产品名称:Recombinant Mouse FGF-21 参考文献: 1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.<br/>2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.<br/>3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95 氨基酸序列: AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS Accession: <a href="http://www.uniprot.org/uniprot/Q9JJN1" target="_blank">Q9JJN1</a> Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/56636" target="_blank">56636</a> 种属: Murine 纯度: > 97 % by SDS-PAGE and HPLC analyses. 来源: Escherichia coli. 内毒素水平: Less than 1 EU/μg of rMuFGF-21 as determined by LAL method. 活性: Fully biologically active when compared to standard. The ED<sub>50</sub> as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10<sup>3</sup> IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin. 形态: Lyophilized from a 0.2 μm filtered concentrated solution in 3 × PBS, pH 7.4. 溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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