供货周期: | 一周 |
品牌: | 爱必信 |
规格: | 10ug |
货号: | abs04179 |
CAS号: |
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产品描述: 产品名称:Recombinant Mouse Fibroblast Growth Factor 1/FGF-1/FGFa (Phe16-Asp155) 描述: Recombinant Mouse Fibroblast growth factor 1 is produced by our E.coli expression system and the target gene encoding Phe16-Asp155 is expressed. 氨基酸序列: FNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYL AMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAI LFLPLPVSSD 背景说明: FGF acidic is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. 别名: Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Heparin-Binding Growth Factor 1, HBGF-1, Fgf1, Fgf-1, Fgfa Accession: P61148 纯度: Greater than 95% as determined by reducing SDS-PAGE. 来源: Escherichia coli. 内毒素水平: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. 活性: Measured by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin. The ED50 for this effect is typically 0.3-1.8 ng/ml. 形态: Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 500mM NaCl, pH 6.6. 溶解方法: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 储存/保存方法: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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