供货周期: | 一周 |
品牌: | 爱必信 |
规格: | 10ug |
货号: | abs04424 |
CAS号: |
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产品描述: 产品名称:Recombinant Human UFM1-Activating Enzyme 5/UBA5 (N-6His) 描述: Recombinant Human Ubiquitin-fold Modifier 1 Activating Enzyme is produced by our E.coli expression system and the target gene encoding Met1-Met404 is expressed with a 6His tag at the N-terminus. 氨基酸序列: MGSSHHHHHHSSGLVPRGSHMAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSS EVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVEL ANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPV DLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDD RNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITV AYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM 背景说明: UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2. 别名: Ubiquitin-like modifier-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBE1DC1, UBA5 Accession: Q9GZZ9 纯度: Greater than 95% as determined by reducing SDS-PAGE. 来源: Escherichia coli. 内毒素水平: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. 形态: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl pH 8.0,1mM DTT, 10% glycerol, 50mM NaCl.
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