Annexin V-PE/7-AAD apoptosis analysis kit|25T|abs50007-爱必信(上海)生物科技有限公司

Annexin V-PE/7-AAD apoptosis analysis kit

参考价：¥1764

供货周期：

一周

品牌：

爱必信

规格：

25T

货号：

abs50007

CAS号：

产品详情

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产品描述：

产品名称：Annexin V-PE/7-AAD apoptosis analysis kit

描述:

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining.

Products List：Annexin V-PE、7-AAD Viability Staining solution、Annexin V Binding Buffer、Apoptosis Positive Control Solution。

储存/保存方法: Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months.

应用: Each lot of this reagent is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used 5 μl/10^5 cells). Since applications vary, the appropriate dilutions must be determined for individual use.

使用方法: Suggested Staining Protocol： 1. Harvest cell (about 1×10⁶cells per test) then wash with cold PBS. 2. Suspend cells in 1 mL 1× Binding Buffer, 300×g centrifugation for 10 minutes, then remove the Binding Buffer from the cell pellet. 3. Resuspend cells in 1 mL 1× Binding Buffer ,adjust cell concentration to 1×10⁶cells/mL. 4. Add 100 μL of cells (1×10⁵cells) to each labeled tube. 5. Add 5 μL of Annexin V-PE to appropriate tubes. 6. Gently vortex each tube and incubate for 10 minutes in room temperature, protected from light. 7. for 5min in room temperature, protected from light. 8. Add PBS to 500μL and vortex gently. 9. Analyze by flow cytometry in 1 hour.

产品信息订购：

产品货号	产品名称	规格	价格	大包装及货期
abs50007	Annexin V-PE/7-AAD apoptosis analysis kit	25T	1764.00	立即咨询
abs50007	Annexin V-PE/7-AAD apoptosis analysis kit	100T	4221.00	立即咨询

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