Ni Sepharose 6 Fast Flow  His标签蛋白纯化填料(镍柱纯化填料)

Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)

参考价:¥750
供货周期: 一周
品牌: 爱必信
规格: 10ml
货号: abs9903
CAS号:
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产品描述:

产品名称:Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)

描述:

Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.

In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.

In research scale applications: This resin binds 40 mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.

Meets industrial demands with security of supply, robust performance, and regulatory support.

Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.

Excellent flow properties provide great performance even at high flow rates.

High binding capacity resin with excellent flow properties

Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.

 

Chromatography technique

色谱技术

His tidine-tagged protein purification 

BioProcess resin

生物工艺树脂

Yes 

Particle size, d50V

粒径

~90 μm 

Matrix

矩阵

6% cross-linked agarose 

Particle Size

粒径

45 μm-165 μm 

Binding Capacity/ml Chromatography Medium

结合力/ ml色谱介质

Approx. 40 mg histidine-tagged protein 

Metal Ion Capacity

金属离子容量

~15 μmol Ni2+/ml medium 

pH stability, operational 

PH稳定性operational 

注:树脂可以在不明显改变功能的情况下操作的pH范围

3–12 

pH stability, CIP3

PH稳定性 CIP3

注:Ni2?剥离介质

2–14 

Pressure/Flow Specification

压力/流量规格

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm 

Storage

保存方法

4 to 30°C, 20% Ethanol 

Chemical stability

化学稳定性

注:Ni2?剥离介质

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

absin特供Cytiva分装(原GE healthcare)分装



产品信息订购:

  产品货号   产品名称   规格 价格 大包装及货期
  abs9903   Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)   10ml   750.00   立即咨询
  abs9903   Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)   25ml   1615.00   立即咨询
  abs9903   Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)   100ml   5560.00   立即咨询
  abs9903   Ni Sepharose 6 Fast Flow His标签蛋白纯化填料(镍柱纯化填料)   500ml   25000.00   立即咨询

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