染料法抗体型 qPCR Mix

2.产品列表

3. 产品特点

4. 实验数据 

1） A301 标准曲线检测

以 Hela 细胞 cDNA 稀释按 10 倍浓度为梯度进行稀释，并以检测 CT 值构建标准曲线。可见使用 A301 扩增效率高、标准曲线线性范围宽、重复性好。 

2） 各种典型模板

A301 进行各种典型模板测试，扩增灵敏度高、特异性好。

3） A301 复杂模板检测

以 Hela 细胞为模板，检测 High-GC 基因（G+C=72%）扩增效果。可见对于 A301 检测复杂模 板扩增不受影响，且扩增特异性好。 

5. 客户案例 

客户单位：兰州大学生科院 细胞所。 

材料：马铃薯 RNA 逆转录的 cDNA，5 倍稀释液。 为了验证引物的特异性，使用 16 个样本检测熔解曲线。具体如下，扩增反应完成后，通过逐渐增加温度 同时监测每一步的荧光信号来产生熔解曲线。结果显示 2 个引物均为单峰，特异性较好。

扩增曲线如下：

6. 引用文献 

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2. Lihui Lu, Yuqi Ren, Tao Yu, et al. Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus.  NAT COMMUN. 2020, 11, 262. IF: 12.19 

3. Le Li, Youxiang Mao, Lina Zhao, et al. p53 regulation of ammonia metabolism through urea cycle controls polyamine  biosynthesis. Nature. volume 567, pages253–256(2019). Doi.org/10.1038/s41586-019-0996-7 IF: 41.577 

4. Yao Wang, Shaochun Yuan, Xin Jia, et al. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral  responses through MAVS. Nat Cell Biol. 2019.21:1346–1356 IF: 20.06 

5. Tao Liu, Yutao Zhou, Liqiu Wang, Liyue Li, Qing Bao, Shuo Tian, Meixin Chen, Hexin Chen, Jun Cui and Chunwei Li. NOD-like  receptor family, pyrin domain containing 3 (NLRP3) contributes to inflammation, pyroptosis, and mucin production in human  airway epithelium on rhinovirus infection. J Allergy Clin Immunol. 2019.144(3):777-787. IF: 14.11 

6. Yong Tang, Hailin Tu, Jie Zhang, et al. K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during  embryogenesis and inflammation. NAT COMMUN. 2019; 10: 4157. IF: 12.19 

7. Le Li, Lijia Li, Wei Li, et al. TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell  proliferation. Nature Communications. (2018) IF: 12.353 

8. Yifei Zhang, Hua Rong, FangXiong Zhang, et al. A Membrane Potential- and Calpain-Dependent Reversal of Caspase-1 Inhibition  Regulates Canonical NLRP3 Inflammasome. Cell Reports. Volume 24, Issue 9, 28 August 2018. Doi:  10.1016/j.celrep.2018.07.098 IF: 8.032 

9. Yina Jiang, Qiujin Xie, Wanxiao Wang, et al. Medicago AP2-domain Transcription Factor WRI5a Is a Master Regulator of Lipid  Biosynthesis and Transfer During Mycorrhizal Symbiosis. Molecular Plant. Volume 11, Issue 11, 5 November 2018, Pages  1344-1359. IF: 9.326 

10. Qingxiang Liu, Yaoxing Wu, Yunfei Qin, et al. Broad and diverse mechanisms used by deubiquitinase family members in  regulating the type I interferon signaling pathway during antiviral responses. Science Advances. 02 May 2018: Vol. 4, no.  5, eaar2824. DOI: 10.1126/sciadv.aar2824 IF: 11.511 

11. Kunpeng Liu, Chuanxia Zhang, Bowen Li, et al. Mutual Stabilization between TRIM9 Short Isoform and MKK6 Potentiates p38  Signaling to Synergistically Suppress Glioblastoma Progression. Cell Reports. Volume 23, Issue 3, 17 April 2018, Pages 838- 851. IF: 8.032 

12. Shouheng Jin, Xiya Zhang, Yanyan Miao, et al. m6A RNA modification controls autophagy through upregulating ULK1 protein  abundance. Cell Res. 2018. 28:955–957 IF: 17.848 

13. Yang Du, Tianhao Duan, Yanchun Feng, et al. LRRC25 inhibits type I IFN signaling by targeting ISG15-associated RIG-I for  autophagic degradation. EMBO Journal, 2018.37(3):351-366. IF: 11.227 

14. Yanyan Zheng, Qingxiang Liu, Yaoxing Wu, et al, Zika virus elicits inflammation to evade antiviral response by cleaving cGAS  via NS1-caspase-1 axis. EMBO J. 2018 Sep 14;37(18). IF: 11.227 

15. Shouheng Jin, Shuo Tian, Man Luo, et al. Tetherin Suppresses Type I Interferon Signaling by Targeting MAVS for NDP52- Mediated Selective Autophagic Degradation in Human Cells. Molecular Cell. Volume 68, Issue 2, 19 October 2017, Pages  308-322.e4. Doi: 10.1016/j.molcel.2017.09.005 IF: 14.248 

16. Yina Jiang, Wanxiao Wang, Qiujin Xie, et al. Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic  fungi. Science 08 Jun 2017, DOI: 10.1126/science. aam9970. IF: 37.205 

17. Peiwen Lu, Changming Shi, Hai Qi; Ephrin B1–mediated repulsion and signaling control germinal center T cell territoriality and function. Science 19 May 2017: Vol. 356, Issue 6339, DOI: 10.1126. IF: 37.205 

18. Wang C, Wang G, Zhang C, et al. OsCERK1-mediated chitin perception and immune signaling requires Receptor-like  Cytoplasmic Kinase 185 to activate a MAPK cascade in rice. Mol Plant. 2017, doi: 10.1016/j.molp.2017.01.006. IF: 8.827 

19. Huifang Xian, Weihong Xie, Shuai Yang, et al. Stratified ubiquitination of RIG-I creates robust immune response and induces  selective gene expression. Science Advances. 22 Sep 2017. DOI: 10.1126/sciadv.1701764 IF: 11.511 

20. Yunfei Qin, Zexiong Su, Yaoxing Wu, et al. NLRP11 disrupts MAVS signalosome to inhibit type I interferon signaling and virusinduced apoptosis. EMBO reports (2017) 18, 2160-2171. DOI 10.15252/embr.201744480 IF: 8.749 

21. Yunfei Qin, Qingxiang Liu, Shuo Tian, et al; TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production  of type I interferon by recruiting GSK3β to TBK1. Cell Research 26, 613-628 (May 2016). IF: 14.812 

22. Xiaonan Liu, Xiaolu Qu, Yuxiang Jiang, et al. Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube  Growth. Molecular Plant. 2015, Pages 1694-1709. https://doi.org/10.1016/j.molp.2015.09.013 IF: 7.142