型号: | NTA |
产地: | 德国 |
品牌: | 库柏生物 |
评分: |
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PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品,组氨酸标签是应用广泛的亲和层析标签,这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性,NTA相对于IDA具有更高的洗脱液纯度。
产品优势:
1)德国制造,品质与信誉的坚实保证;
2)结合载量可高达80mg/mL,目前市面上高结合能力;
3)更优越的DTT和EDTA稳定性和耐受性;
4)凝胶的多孔性和均匀粒径,使蛋白与凝胶之间有很好地相互作用;
5)适合低压环境下的纯化过程(大规模纯化制备或预装柱均可使用);
6)高性价比,为研发、生产降低成本;
7)适用于大肠杆菌和真核细胞的裂解液和上清液;
8)同时可装载Co, Cu, Zn和其它金属离子。
优势详解:
1. 收率高,纯度高
Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).
Cube Biotech | |
蛋白结合能力 | > 80mg/mL |
2. 优越的DTT和EDTA稳定性
Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实,在稳定性测试中,将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His,凝胶的蛋白结合能力因存在DTT与EDTA而下降,但是衰减率平缓,即使在1.5 mM EDTA,凝胶依然具有其大结合能力的54%。
Cube Biotech | |
DTT稳定性 | <10mM |
EDTA稳定性 | <1.5mM |
应用 | Specific binding and purification of 6x his-tagged proteins |
特异性 | Affinity to His-tagged proteins |
结合能力 | >80?mg/mL |
Bead Ligand | Ni-NTA |
粒径大小 | 40 μm |
螯合剂稳定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50?% suspension |
所需试剂耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50?mL tube (min 10,000 x g) 50?mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml ?pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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订货信息:
31101 | PureCube Ni-NTA Agarose (1 ml) |
31103 | PureCube Ni-NTA Agarose (10 ml) |
31105 | PureCube Ni-NTA Agarose (50 ml) |
31110 | PureCube Ni-NTA Agarose (250 ml) |
31112 | PureCube Ni-NTA Agarose (500 ml) |
31115 | PureCube Ni-NTA Agarose (1000 ml) |
31120 | PureCube Ni-NTA Agarose (5000 ml) |
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