Anti-IDH2(R172K) Mouse Monoclonal Antibody/现货抗体2023更新文献| 100 微升|26163-武汉费斯德生物科技有限公司

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Anti-IDH2(R172K) Mouse Monoclonal Antibody/现货抗体2023更新文献

货号: 26163

基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

描述: Anti-IDH2(R172K) Mouse Monoclonal Antibody

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背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas.

免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin

经过测试的应用: ELISA, WB, IHC

推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgG

纯度: Purified from ascites

防腐剂: No

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: recognizes R172K mutant, but not wild-type IDH2 of vertebrates

储存条件: Store at -20°C. Avoid repeated freezing and thawing

Anti-IDH2(R172K) Mouse Monoclonal Antibody/现货抗体2023更新文献

蛋白质印迹: Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163). 免疫荧光: Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163).

Anti-IDH2(R172K) Mouse Monoclonal Antibody/现货抗体2023更新文献

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企业名称: 武汉费斯德生物科技有限公司
企业类型: 有限责任公司(自然人独资)
信用代码: 91420103MA7H14773E
成立日期: 2022年01月19日
注册资本: 壹佰万圆整
经营范围: 一般项目:细胞技术研发和应用;生物化工产品技术研发;工程和技术研究和试验发展;自然科学研究和试验发展;医学研究和试验发展(除人体干细胞、基因诊断与治疗技术开发和应用);技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;销售代理;实验分析仪器销售;货物进出口;技术进出口;进出口代理;化工产品销售(不含许可类化工产品)(除许可业务外,可自主依法经营法律法规非禁止或限制的项目)

供货周期：	现货
品牌：	美国Neweast Biosciences
规格：	100 微升
货号：	26163
CAS号：