AMV Reverse Transcriptase, 10u/ul
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普瑞麦迪(北京)实验室技术有限公司
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Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+.






































Cat No
Size
Price($)


A1201-200
200 units
75


A1201-500
500 units
155















 

 




















The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.


 


Applications

- First- and second-strand synthesis of cDNA.

- Primer extensions and RNA sequencing

- RT-PCR(a). Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase.


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