产品详情
产品名称: 鸡甲状旁腺激素(PTH)ELISA Kit
英文名称: Chicken Parathyroid hormone,PTH ELISA Kit
货号: CSB-E11880Ch
规格: 96T
种属: Chicken
待测物名称: parathyroid hormone
缩写: PTH
样本类型: serum, plasma, tissue homogenates
检测范围: 6.25pg/ml-100pg/ml
灵敏度: 3.125pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
鸡甲状旁腺激素(PTH)ELISA Kit
用途: For research use only. Not for diagnostic use.
鸡甲状旁腺激素(PTH)ELISA Kit
原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
鸡甲状旁腺激素(PTH)ELISA Kit
特异性: This assay has high sensitivity and excellent specificity for detection of Chicken PTH. No significant cross-reactivity or interference between Chicken PTH and analogues was observed.
鸡甲状旁腺激素(PTH)ELISA Kit
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
鸡甲状旁腺激素(PTH)ELISA Kit
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
鸡甲状旁腺激素(PTH)ELISA Kit
检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
鸡甲状旁腺激素(PTH)ELISA Kit
结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PTH concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
鸡甲状旁腺激素(PTH)ELISA Kit
相关产品:
产品名称 英文名称 货号 规格
小鼠甲状旁腺激素(PTH)ELISA kit Mouse parathyroid hormone (PTH) ELISA kit CSB-E08690m 96T
大鼠甲状旁腺激素(PTH) ELISA Kit Rat Parathyroid hormone,PTH ELISA Kit CSB-E07866r 96T
猴子甲状旁腺激素(PTH)ELISA Kit Monkey Parathyroid hormone,PTH ELISA Kit CSB-E14299Mk 96T
人甲状旁腺激素(PTH)ELISA Kit human Parathyroid hormone,PTH ELISA Kit CSB-E06934h 96T
鸡甲状旁腺激素(PTH)ELISA Kit
兔甲状旁腺激素(PTH)ELISA Kit Rabbit Parathyroid hormone,PTH ELISA Kit CSB-E13244Rb 96T
Pig Parathyroid hormone(PTH) ELISA kit Pig Parathyroid hormone(PTH) ELISA kit CSB-EL018987PI 96T
Horse Parathyroid hormone(PTH) ELISA kit Horse Parathyroid hormone(PTH) ELISA kit CSB-EL018987HO 96T
鸡甲状旁腺激素(PTH)ELISA Kit
山羊甲状旁腺激素(PTH)ELISA Kit Goat Parathyroid Hormone(PTH) ELISA kit CSB-E13082G 96T
犬甲状旁腺激素(PTH) ELISA Kit Canine Parathyroid hormone(PTH) ELISA Kit CSB-E17575c 96T
猫甲状旁腺激素(PTH)ELISA Kit Cat Parathyroid hormone,PTH ELISA Kit CSB-E13485Fe 96T
Bovine Parathyroid hormone(PTH) ELISA kit Bovine Parathyroid hormone(PTH) ELISA kit CSB-EL018987BO 96T
鸡甲状旁腺激素(PTH)ELISA Kit
抗体
产品名称 货号 规格
Rabbit anti-human parathyroid hormone polyclonal Antibody CSB-PA018987GA01HU 150ul
鸡甲状旁腺激素(PTH)ELISA Kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Mycobacterium abscessus Peptidyl-tRNA hydrolase(pth) CSB-EP544520MVF 1mg E.coli
Recombinant Mycobacterium abscessus Peptidyl-tRNA hydrolase(pth) CSB-YP544520MVF 1mg Yeast
Recombinant Pseudomonas putida Peptidyl-tRNA hydrolase(pth) CSB-EP544181FGC 1mg E.coli
Recombinant Pseudomonas putida Peptidyl-tRNA hydrolase(pth) CSB-YP544181FGC 1mg Yeast
Recombinant Thermotoga sp. Peptidyl-tRNA hydrolase(pth) CSB-EP543972TNM 1mg E.coli
Recombinant Thermotoga sp. Peptidyl-tRNA hydrolase(pth) CSB-YP543972TNM 1mg Yeast
Recombinant Peptidyl-tRNA hydrolase(pth) CSB-EP543505EVG 1mg E.coli
Recombinant Peptidyl-tRNA hydrolase(pth) CSB-YP543505EVG 1mg Yeast
Recombinant Escherichia coli Peptidyl-tRNA hydrolase(pth) CSB-EP542673ENX 1mg E.coli