产品详情
产品名称：Protein G Sepharose Column
产 地：BioVision
产品货号：6518-1
产品规格：1 ml
产品说明：
Storage Temp.:+4℃
Contents:
Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide.
Features:
High binding capacity = Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
Minimal Leaching of the Ligand
Flow Rate Tested* = 2.07 ml/min.
*Test condition: = Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Description:
Protein G-Sepharose Columns are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. BioVision Protein G (Cat. # 6510) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose Column is >20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose Columns display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
产 地：BioVision
产品货号：6518-1
产品规格：1 ml
产品说明：
Storage Temp.:+4℃
Contents:
Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide.
Features:
High binding capacity = Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
Minimal Leaching of the Ligand
Flow Rate Tested* = 2.07 ml/min.
*Test condition: = Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Description:
Protein G-Sepharose Columns are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. BioVision Protein G (Cat. # 6510) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose Column is >20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose Columns display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
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