2-硝基苯基-β-D-吡喃半乳糖苷

Product Name: 2-Nitrophenyl β-D-galactopyranoside

别名: 2-硝基苯基-β-D-吡喃半乳糖苷(ONPG) ;邻硝基苯-β-D-吡喃半乳糖苷

Cas号: 369-07-3

分子式: C12H15O8N

分子量: 301.25

EINECS编号: 206-716-1

MDL号: MFCD00063255

 N109040-1g 99%，非动物源

 N109040-25g 99%，非动物源

 N109040-5g 99%，非动物源

2-硝基苯基-β-D-吡喃半乳糖苷介绍: 溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。

用途: 用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。

包装: N109040-25gglass btl

N109040-1gglass btl

N109040-5gpoly btl

熔点: 195℃

特定比重: 1.599

比旋光度: -68.8 ° (C=1, H2O)

贮存: -20℃充氩保存

2-硝基苯基-β-D-吡喃半乳糖苷生化和生理学机理: β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.