酪氨酰DNA磷酸二酯酶1(TDP1)重组蛋白

[ PROPERTIES ]

Residues: Met1~Leu271

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q4G056

Host: E. coli

Subcellular Location: Nucleus. Cytoplasm.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.1

Predicted Molecular Mass: 60.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37ºC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

MSQESSYGKW TISSSDDSED EKPKPVKPST SSGPQAGQGV SKEPTYTCSE ARKAAHKRQISPVKF NNT DS VLP HKKQKM D SPEG LGW C LS SS D DEQQP DV TQ QEQPKGVPPQEKKYAPSA NVTTAQKVED RSPPDSHRAQ RADEEYETSG EGQDIWDMLDKENPFQFYLTRVSGIKAKYN SKALHIKDIL SPLFGTLVSS AQFNYCFDVN WLIKQYPPEF RKKPILLVHGDKREAKADLH AQAKPYANIS LCQAKLDIAF GTHHTKMMLL L

[  IDENTIFICATION  ]

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