产品详情
总胆红素、直接胆红素检测检测原理:试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被大鼠谷胱甘肽-过氧化物酶(GSH-PX)获抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤总胆红素、直接胆红素检测用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的大鼠谷胱甘肽-过氧化物酶(GSH-PX)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。
总胆红素、直接胆红素检测需要提供的试剂和器材
37℃恒温箱
标准规格酶标仪
精密移液器及一次性吸头
蒸馏水
一次性试管
吸水纸
总胆红素、直接胆红素检测按试剂组成分类
一、单试剂
①优点:操作简便适用于各类生化分析,节约试剂位。
②缺点:配方复杂(稳定剂),稳定性,不能完全避免内外源物质干扰。
二、双试剂
①优点:可较彻底排除样品空白和内外源干扰物,试剂稳定,便于储存,运输和使用,可用抑制法直接测定某些同工酶。
②缺点:多占试剂位。
更多产品引荐:A-01032 ?Anti-ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1)
A-01033 ?Anti-ABL2/ARG(v-abl Abelson murine leukemia viral oncogene homolog 2)
A-01034 ?Anti-ABI1/SSH3BP1(Abelson interactor 1)
A-01035 ?Anti-ABP(Androgen Binding Protein)
A-01036 ?Anti-ACE1( ACE, testis-specific isoform precursor)
A-01037 ?Anti-ACE2(Angiotensin converting enzyme 2)
A-01038 ?Anti-ACEI (Angiotensin I Converting Enzyme Inhibitor )
A-01039 ?Anti-ACD/PTOP(Adrenocortical dysplasia protein)
A-01040 ?Anti-Acinus (Apoptotic chromatin condensation Inducer in the nucleus)
A-01041 ?Anti-Ack1
A-01042 ?Anti-ACTH (Adrenorticotrophin hormons 1-39)
A-01043 ?Anti-ACTH (Adrenorticotrophin hormons 18-39)
A-01044 ?Anti-ACTH (Adrenorticotrophin hormons 7-23)
A-01045 ?Anti-Actin α /α-SMA (Actin alpha , smooth muscle aorta)
A-01046 ?Anti-α-ACTN4(α-Actinin 4)
A-01047 ?Anti-F-Actin(filament actin)
A-01048 ?Anti-AD7C-NTP/NTP/AF (Neurual thread protein)
A-01049 ?Anti-AD7C-NTP/NTP/AF (Neurual thread protein)human
A-01050 ?Anti-AD7C-NTP/NTP/AF(Neurual thread protein)
A-01051 ?Anti-ADAM-TS1/METH1(a disintegrin and metalloproteinase with-thrombospodin type 1)
A-01052 ?Anti-ADAM-TS7 (A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 7)
A-01053 ?Anti-ADAM-TS7 (A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 7)
A-01054 ?Anti-ADAM-TS12(A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 12)
A-01055 ?Anti-Adducin protein
A-01056 ?Anti-ADFP/ADRP/adipophilin (adipose differentiation-related protein)
A-01057 ?Anti-AEV(Avian Encephalomyelitis virus)
A-01058 ?Anti-ADH/AVP(Antidiuretic Hormone;Arginine Vasopressin)
A-01059 ?Anti-Adipo R1 (adiponectin receptor 1)
A-01060 ?Anti-Adipo R2 (adiponectin receptor 2)
A-01061 ?Anti-Adiponectin
A-01062 ?Anti-ADM/AM (Adrenomedullin)
A-01063 ?Anti-ADM (Adrenomedullin)ProAM-N20
A-01064 ?Anti-ADM R (Adrenomedullin receptor)
A-01065 ?Anti-ADNP/NAP (Activity-dependent Neuroprotective protein )