产品详情
Eukaryotic Aspartate Aminotransferase (AST)
天冬氨酸转氨酶(AST)真核蛋白
[ PROPERTIES ]
Source: Eukaryotic expression
Host: Yeast
Residues: Ala2~Gln413
Tags: N-terminal His Tag
Homology: Mouse 90%, Rat 90%
Tissue Specificity: Kidney, stomach, liver, intestine.
Subcellular Location: Cytoplasm
Purity: > 95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 5%Trehalose and Proclin300.
Original Concentration: 200µg/mL
Predicted isoelectric point: 6.6
Predicted Molecular Mass: 47.5kDa
Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducingconditions.
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays;Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
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