Eukaryotic Osteoprotegerin (OPG)

骨保护素(OPG)真核蛋白

[ PROPERTIES ]

Source: Eukaryotic expression

Host: 293F Cell

Residues: Tyr31~Leu401

Tags: N-terminal His Tag

Homology: Mouse 85%, rat 85%

Tissue Specificity: Heart, kidney, liver, spleen, pancreas.

Subcellular Location: Secreted

Purity: > 95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.7

Predicted Molecular Mass: 42.4kDa

Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays;Purification; Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[ IDENTIFICATION ]

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