Recombinant Transcriptional Intermediary Factor 1 Beta (TIF1b)

转录中介因子1β(TIF1b)重组蛋白

[ PROPERTIES ]

Residues: Pro504~Ile785

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q13263

Host: E. coli

Subcellular Location: Nucleus

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative..

Predicted isoelectric point: 4.6

Predicted Molecular Mass: 33.1kDa

Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37ºC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

PVFKVFP GSTTEDYNLI VIERGAAAAA TGQPGTAPAG TPGAPPLAGMAIVKEEETEAAIGAPPTATE GPETKPVLMA LAEGPGAEGP RLASPSGSTS SGLEVVAPEGTSAPGGGPGTLDDSATICRV CQKPGDLVMC NQCEFCFHLD CHLPALQDVP GEEWSCSLCH VLPDLKEEDGSLSLDGADST GVVAKLSPAN QRKCERVLLA LFCHEPCRPL HQLATDSTFS LDQPGGTLDLTLIRARLQEK LSPPYSSPQE FAQDVGRMFK QFNKLTEDKA DVQSI

[  IDENTIFICATION  ]

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