Recombinant Lumican (LUM)

基膜聚糖(LUM)重组蛋白

[ PROPERTIES ]

Residues: Gln19~Asn338,with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted, extracellularspace, extracellular matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 38.1kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37ºC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-QY YDYDIPLFMY GQISPNCAPE CNCPHSYPTA MYCDDLKLKSVPMVPPGIKY LYLRNNQIDH IDEKAFENVT DLQWLILDHN LLENSKIKGK VFSKLKQLKKLHINYNNLTE SVGPLPKSLQ DLQLTNNKIS KLGSFDGLVN LTFIYLQHNQLKEDAVSASLKGLKSLEYLD LSFNQMSKLP AGLPTSLLTL YLDNNKISNI PDEYFKRFTGLQYLRLSHNELADSGVPGNS FNISSLLELD LSYNKLKSIP TVNENLENYY LEVNELEKFD VKSFCKILGPLSYSKIKHLR LDGNPLTQSS LPPDMYECLR VANEITVN

[  IDENTIFICATION  ]

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