钙调蛋白1(CALM1)重组蛋白

[ DESCRIPTION ]

Protein Names: Calmodulin 1

Synonyms: CALM1, CALML2, CAM3, CAMC, CAMIII

Species: Human

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Cytoplasm, cytoskeleton, spindle. spindle pole.

[ PROPERTIES ]

Residues: Met1~Lys149, with two N-terminal Tags, His-tag andGST-tag.

Grade & Purity: >95%, 44 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg

(determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 43.6 kDa

Predicted isoelectric point: 4.9.

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.

MRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTLEVLFQGPLGS-MADQLTEEQI AEFKEAFSLFDKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGYISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

[  IDENTIFICATION  ]

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考！