成纤维细胞生长因子内结合蛋白(FIBP)重组蛋白

[ PROPERTIES ]

Residues: Leu14~Met226

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O43427

Host: E. coli

Subcellular Location: Nucleus. Peripheral Membrane Protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.1

Predicted Molecular Mass: 28.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37ºC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

LIDEDVY RLWLDGYSVT DAVALRVRSG ILEQTGATAA VLQSDTMDHY RTFHMLERLLHAPPKLLHQL IFQIPPSRQA LLIERYYAFD EAFVREVLGK KLSKGTKKDL DDISTKTGITLKSCRRQFDN FKRVFKVVEE MRGSLVDNIQ QHFLLSDRLA RDYAAIVFFA NNRFETGKKKLQYLSFGDFA FCAELMIQNW TLGAVGEAPT DPDSQM

[  IDENTIFICATION  ]

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