免疫球蛋白G(IgG)真核蛋白

[ PROPERTIES ]

Source: Eukaryotic expression

Host: 293F cell

Residues: Met1~Cys238

Tags: N-terminal His Tag

Tissue Specificity: Serum.

Subcellular Location: Secreted

Purity: > 95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 27.0kDa

Accurate Molecular Mass: 55&25kDa as determined by SDS-PAGEreducingconditions.

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays;Purification; Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Phenomenon explanation:

Rabbit IgG consists of 1455 amino acids and has a predicted molecular massof160 kDa. As a result of disulfide bond, the apparent molecular mass of IgGisapproximately two lines 55kDa heavy chain and two lines 25kDa light chaininSDS-PAGE under reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no

obvious degradation and precipitation were observed. The loss rate is less than 5% within the

expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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