2-硝基苯基-β-D-吡喃半乳糖苷
99%，非动物源
Product Name: 2-Nitrophenyl β-D-galactopyranoside
| 别名: | 2-硝基苯基-β-D-吡喃半乳糖苷(ONPG) ;邻硝基苯-β-D-吡喃半乳糖苷 |
| CAS号: | 369-07-3 |
| 分子式: | C12H15O8N |
| 分子量: | 301.25 |
| EINECS编号: | 206-716-1 |
| MDL号: | MFCD00063255 |
2-硝基苯基-β-D-吡喃半乳糖苷
| 介绍: | 溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。 |
| 用途: | 用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。 |
| 熔点: | 195℃ |
| 特定比重: | 1.599 |
| 比旋光度: | -68.8 ° (C=1, H2O) |
| 贮存: | -20℃充氩保存 |
| 生化和生理学机理: | β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
2-硝基苯基-β-D-吡喃半乳糖苷
