产品详情
Product Name: CHAPS
别名: | 3-[(3-胆固醇氨丙基)二甲基氨基]-1-丙磺酸 ;3-[3-(胆酰胺丙基)二甲氨基]丙磺酸内盐 |
CAS号: | 75621-03-3 |
分子式: | C32H58N2O7S |
分子量: | 614.88 |
MDL号: | MFCD00012116 |
介绍: | www.aladdin-reagent.com 用于变性裂解细胞;提取RNA和DNA,无RNA酶和DNA酶活性 |
用途: | 两性表面活性剂,CHAPS 是一种非变性的zwitterionic 去垢剂、蛋白质裂解液,用于溶解膜蛋白和裂解蛋白和蛋白之间的相互作用。 |
特定比重: | 1.01 |
贮存: | RT |
生化和生理学机理: | CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM. |