人细胞周期素D1(CCND1)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人细胞周期素D1(CCND1)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人细胞周期素D1(CCND1)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人细胞周期素D1(CCND1)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人细胞周期素D1(CCND1)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1606c 人细胞外基质金属蛋白酶诱导因子(EMMPRIN/CD147)ELISA试剂盒 Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit

XY-EL-H1607c 人CD34分子(CD34)ELISA试剂盒 Human CD34 (Cluster Of Differentiation 34) ELISA Kit

XY-EL-H1609c 人抗小核糖核蛋白/Sm抗体(snRNP/Sm)ELISA试剂盒 Human snRNP/Sm (Small Nuclear Ribonucleoprotein) ELISA Kit

XY-EL-H1610c 人细胞色素b5(CYB5A)ELISA试剂盒 Human CYB5A (Cytochrome b5) ELISA Kit

XY-EL-H1611c 人绒毛膜促性腺激素β肽(CGβ)ELISA试剂盒 Human CGβ (Chorionic Gonadotropin Beta Polypeptide) ELISA Kit

XY-EL-H1612c 人可溶性OX40L蛋白(sOX40L)ELISA试剂盒 Human sOX40L (soluble OX40L) ELISA Kit

XY-EL-H1613c 人抗原提呈相关转运蛋白(TAP)ELISA试剂盒 Human TAP (Transporter Associated with Antigen Processing) ELISA Kit

XY-EL-H1614c 人B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)ELISA试剂盒 Human BRAF (B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit

XY-EL-H1615c 人分泌型ST2(sST2)ELISA试剂盒 Human sST2 (soluble ST2)ELISA Kit

XY-EL-H1616c 人颗粒酶A(GzmA)ELISA试剂盒 Human GzmA (Granzyme A) ELISA Kit

XY-EL-H1617c 人颗粒酶B(GzmB)ELISA试剂盒 Human GzmB (Granzyme B) ELISA Kit

XY-EL-H1618c 人颗粒溶素(GNLY)ELISA试剂盒   Human GNLY (Granulysin) ELISA Kit

XY-EL-H1619c 人颗粒体蛋白(GRN)ELISA试剂盒 Human GRN (Granulin) ELISA Kit

XY-EL-H1620c 人S100钙结合蛋白P(S100P)ELISA试剂盒 Human S100P (S100 Calcium Binding Protein P) ELISA Kit

XY-EL-H1621c 人补体受体2 (CR2/CD21)ELISA试剂盒 Human CR2/CD21 (Complement Receptor 2) ELISA Kit

XY-EL-H1622c 人白细胞分化抗原28(CD28)ELISA试剂盒 Human CD28 (Cluster of Differentiation 28) ELISA Kit 

XY-EL-H1623c 人白细胞分化抗原30配体(CD30L)ELISA试剂盒 Human CD30L (Cluster of Differentiation 30 Lligand) ELISA Kit 

XY-EL-H1624c 人环腺苷二磷酸核糖水解酶(cADPRH/CD38)ELISA试剂盒 Human cADPRH/CD38 (Cyclic ADP Ribose Hydrolase) ELISA Kit

XY-EL-H1625c 人游离前列腺特异性抗原(F-PSA)ELISA试剂盒 Human F-PSA (Free Prostate-Specific Antigen) ELISA Kit

XY-EL-H1626c 人B淋巴细胞激活抗原B7-2(LAB7-2/CD86)ELISA试剂盒 Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit

XY-EL-H1627c 人绒毛膜促性腺激素β肽(CGβ)ELISA试剂盒 Human CGb (Chorionic Gonadotropin Beta Polypeptide) ELISA Kit

XY-EL-H1628c 人可溶性半乳糖凝集素3(LGALS3)ELISA试剂盒 Human LGALS3 (Lectin-Galactoside Binding Soluble 3) ELISA Kit

XY-EL-H1629c 人表皮生长因子受体2(EGFR2)ELISA试剂盒 Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit

XY-EL-H1630c 人封闭蛋白5(CLDN5)ELISA试剂盒 Human CLDN5 (Claudin 5) ELISA Kit

XY-EL-H1631c 人N-甲基-D-天氡氨酸离子能谷氨酸受体2B(GRIN2B)ELISA试剂盒 Human GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B) ELISA Kit

XY-EL-H1632c 人N-甲基-D-天氡氨酸离子能谷氨酸受体1(GRIN1)ELISA试剂盒 Human GRIN1 (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit

XY-EL-H1633c 人N-甲基-D-天氡氨酸离子能谷氨酸受体2A(GRIN2A)ELISA试剂盒 Human GRIN2A (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A) ELISA Kit

XY-EL-H1634c 人Frataxin蛋白(FXN)检测试剂盒(酶联免疫吸附试验法) Human FXN (Frataxin) ELISA Kit

XY-EL-H1635c 人凝集素样氧化低密度脂蛋白受体1(LOX-1)ELISA试剂盒 Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit

XY-EL-H1636c 人辅肌动蛋白α4(ACTN4)ELISA试剂盒 Human ACTN4 (Actinin Alpha 4) ELISA Kit

XY-EL-H1638c 人可溶性腺苷酸环化酶(sAC)ELISA试剂盒 Human sAC (Soluble Adenylate Cyclase) ELISA Kit

XY-EL-H1639c 人可溶性血纤蛋白单体复合物(sFMC)ELISA试剂盒 Human sFMC (Soluble Fibrin Monomer Complex) ELISA Kit

XY-EL-H1640c 人血小板内皮细胞粘附分子1(PECAM1/CD31)ELISA试剂盒 Human PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit

XY-EL-H1641c 人XVIII型胶原α1(COL18α1)ELISA试剂盒 Human COL18α1 (Collagen Type XVIII Alpha 1) ELISA Kit

XY-EL-H1642c 人载脂蛋白B48(ApoB48)ELISA试剂盒 Human ApoB48 (Apolipoprotein B48) ELISA Kit