产品详情
小鼠戊糖素(PTD)检测试剂盒免疫标记技术是将一些既易测定又具有高度敏感性的物质标记到特异性抗原或抗体分子上,通过这些标记物的增强放大效应来显示反应系统中抗原或抗体的性质与含量。常用的标记物包括荧光素、酶和放射性核素等,用这3种标记物进行标记的免疫检测技术被称为3大免疫标记技术。目前,使用的免疫标记物还有化学发光物质、铁蛋白和胶体金等。
小鼠戊糖素(PTD)检测试剂盒酶制剂及其底物
凡无毒性又能呈现有色化学反应的酶,原则上均可作为标记用。但作为标记抗体用的酶应满足下列要求:
(1)来源方便,易于纯化;
(2)比活性高,性质稳定;
(3)酶活性和量能
小鼠戊糖素(PTD)检测试剂盒用简单方法测定。目前在免疫酶技术中常用的酶为辣根过氧化物酶(HRP)和硷性磷酸酶(AP),其次还有葡萄糖氧化酶,β-半乳糖苷酶、溶菌酶和苹果酸脱氢酶等。
小鼠戊糖素(PTD)检测试剂盒由于辣根过氧化物酶(Horseradish Peroxidase, HRP)比活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(最高可达3.4)。小鼠戊糖素(PTD)检测试剂盒RZ值越小,非酶蛋白就越多。值得注意的是,纯度并不表示酶活性,如当酶变性后,RZ值仍可不变。
HZ-EL-Ch0306c 鸡垂体腺苷酸环化酶激活肽受体 (PACAPR)检测试剂盒 Chicken PACAPR (Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1) ELISA Kit
HZ-EL-Ch0307c 鸡鸟苷酸交换因子(GEF)检测试剂盒 Chicken GEF (Guanylate Exchange Factor) ELISA Kit
HZ-EL-Ch0308c 鸡孕酮和adipoQ受体家族成员Ⅶ(PAQR7)检测试剂盒 Chicken PAQR7 (Progestin and AdipoQ Receptor Family Member VII) ELISA Kit
HZ-EL-Ch0309c 鸡核孔蛋白214kda(NUP214)检测试剂盒 Chicken NUP214 (Nucleoporin 214kDa) ELISA Kit
HZ-EL-Ch0310c 鸡XC趋化因子受体1(XCR1)检测试剂盒 Chicken XCR1 (XC-Chemokine Receptor 1) ELISA Kit
HZ-EL-Ch0311c 鸡拮抗凋亡转录因子(AATF)检测试剂盒 Chicken AATF (Apoptosis Antagonizing Transcription Factor) ELISA Kit
HZ-EL-Ch0312c 鸡多巴胺转运蛋白(DAT)检测试剂盒 Chicken DAT (Dopamine Transporter) ELISA Kit
HZ-EL-Ch0313c 鸡成纤维细胞生长因子6(FGF6)检测试剂盒 Chicken FGF6 (Fibroblast Growth Factor 6) ELISA Kit
HZ-EL-Ch0314c 鸡细胞角蛋白19(CK-19/KRT19)检测试剂盒 Chicken CK-19/KRT19 (Cytokeratin 19) ELISA Kit
HZ-EL-Ch0315c 鸡神经调节蛋白3(NRG-3)检测试剂盒 Chicken NRG-3 (Neuregulin 3) ELISA Kit
HZ-EL-Ch0316c 鸡肾上腺皮质发育异常蛋白(ACD)检测试剂盒 Chicken ACD (Adrenocortical Dysplasia Homolog) ELISA Kit
HZ-EL-Ch0317c 鸡芳香酶(ARO)检测试剂盒 Chicken ARO (Aromatase) ELISA Kit
HZ-EL-Ch0318c 鸡细胞色素C(Cyt-C)检测试剂盒 Chicken Cyt-C (Cytochrome C) ELISA Kit
HZ-EL-Ch0319c 鸡降钙素(CT)检测试剂盒 Chicken CT (Calcitonin) ELISA Kit
HZ-EL-Ch0320c 鸡可溶性细胞间粘附分子1(sICAM-1)检测试剂盒 Chicken sICAM-1 (Soluble Intercellular Adhesion Molecule-1) ELISA Kit
HZ-EL-Ch0321c 鸡基质金属蛋白酶原1(Pro-MMP-1)检测试剂盒 Chicken Pro-MMP-1 (pro-Matrix Metalloproteinase 1) ELISA Kit
HZ-EL-Ch0322c 鸡血管生成素样蛋白6(ANGPTL6)检测试剂盒 Chicken ANGPTL6 (Angiopoietin Like Protein 6) ELISA Kit
HZ-EL-Ch0323c 鸡GTP酶活化蛋白(GAP)检测试剂盒 Chicken GAP (GTPase Activating Protein) ELISA Kit
HZ-EL-Ch0324c 鸡基质金属蛋白酶组织抑制因子1(TIMP-1)检测试剂盒 Chicken TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
HZ-EL-Ch0325c 鸡蛋白聚糖(PG)检测试剂盒 Chicken PG (Proteoglycan) ELISA Kit
HZ-EL-Ch0326c 鸡乳酸脱氢酶C(LDHC)检测试剂盒 Chicken LDHC (Lactate Dehydrogenase C) ELISA Kit
HZ-EL-Ch0327c 鸡多巴胺受体D4(D4R/DRD4)检测试剂盒 Chicken D4R/DRD4 (Dopamine Receptor D4) ELISA Kit
HZ-EL-Ch0328c 鸡富含半胱氨酸蛋白61(Cyr61)检测试剂盒 Chicken Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
HZ-EL-Ch0329c 鸡胃粘液素(GM)检测试剂盒 Chicken GM (Gastric Mucin) ELISA Kit
HZ-EL-Ch0330c 鸡白细胞分化抗原28(CD28)检测试剂盒 Chicken CD28 (Cluster of Differentiation 28) ELISA Kit