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大鼠粘着斑激酶(FAK)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠粘着斑激酶(FAK)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠粘着斑激酶(FAK)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠粘着斑激酶(FAK)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠粘着斑激酶(FAK)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-R0457c 大鼠骨形态形成蛋白拮抗蛋白1(GREM1)检测试剂盒 Rat GREM1 (GremLin 1) ELISA Kit
HZ-EL-R0458c 大鼠生长分化因子1(GDF1)检测试剂盒 Rat GDF1 (Growth Differentiation Factor 1) ELISA Kit
HZ-EL-R0459c 大鼠生长分化因子2(GDF2)检测试剂盒 Rat GDF2 (Growth Differentiation Factor 2) ELISA Kit
HZ-EL-R0460c 大鼠生长分化因子3(GDF3)检测试剂盒 Rat GDF3 (Growth Differentiation Factor 3) ELISA Kit
HZ-EL-R0461c 大鼠生长分化因子5(GDF5)检测试剂盒 Rat GDF5 (Growth Differentiation Factor 5) ELISA Kit
HZ-EL-R0462c 大鼠生长分化因子9(GDF9)检测试剂盒 Rat GDF9 (Growth Differentiation Factor 9) ELISA Kit
HZ-EL-R0463c 大鼠生长分化因子11(GDF11)检测试剂盒 Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit
HZ-EL-R0464c 大鼠生长分化因子15(GDF15)检测试剂盒 Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit
HZ-EL-R0465c 大鼠生长因子受体结合蛋白10(GRB10)检测试剂盒 Rat GRB10 (Growth Factor Receptor Bound Protein 10) ELISA Kit
HZ-EL-R0466c 大鼠生长因子受体结合蛋白14(GRB14)检测试剂盒 Rat GRB14 (Growth Factor Receptor Bound Protein 14) ELISA Kit
HZ-EL-R0467c 大鼠生长因子受体结合蛋白2(GRB2)检测试剂盒 Rat GRB2 (Growth Factor Receptor Bound Protein 2) ELISA Kit
HZ-EL-R0468c 大鼠生长激素2(GH 2)检测试剂盒 Rat GH2 (Growth Hormone 2) ELISA Kit
HZ-EL-R0469c 大鼠促生长激素释放激素(GHRH)检测试剂盒 Rat GHRH (Growth Hormone Releasing Hormone) ELISA Kit
HZ-EL-R0470c 大鼠甘油三酯(TG)检测试剂盒 Rat TG (Triglyceride) ELISA Kit
HZ-EL-R0472c 大鼠胃泌素(GT)检测试剂盒 Rat GT (Gastrin) ELISA Kit
HZ-EL-R0473c 大鼠结合珠蛋白(HP)检测试剂盒 Rat HP (Haptoglobin) ELISA Kit
HZ-EL-R0474c 大鼠70kDa热休克蛋白4(HSPA4)检测试剂盒 Rat HSPA4 (Heat Shock 70kDa Protein 4) ELISA Kit
HZ-EL-R0475c 大鼠热休克因子1(HSF1)检测试剂盒 Rat HSF1 (Heat Shock Factor 1) ELISA Kit
HZ-EL-R0476c 大鼠热休克蛋白27(HSP-27)检测试剂盒 Rat HSP-27 (Heat Shock Protein 27) ELISA Kit
HZ-EL-R0477c 大鼠热休克蛋白40(HSP-40)检测试剂盒 Rat HSP-40 (Heat Shock Protein 40) ELISA Kit
HZ-EL-R0478c 大鼠热休克蛋白60(Hsp-60)检测试剂盒 Rat HSP-60 (Heat Shock Protein 60) ELISA Kit
HZ-EL-R0479c 大鼠热休克蛋白70(HSP-70)检测试剂盒 Rat HSP-70 (Heat Shock Protein 70) ELISA Kit
HZ-EL-R0480c 大鼠热休克蛋白90(HSP-90)检测试剂盒 Rat HSP-90 (Heat Shock Protein 90) ELISA Kit
HZ-EL-R0481c 大鼠热休克蛋白糖蛋白96(HSP gp96)检测试剂盒 Rat HSP GP96 (Heat Shock Protein Glycoprotein 96) ELISA Kit
HZ-EL-R0482c 大鼠热休克蛋白47(HSP47)检测试剂盒 Rat HSP47 (Heat Shock Protein) ELISA Kit
HZ-EL-R0483c 大鼠热休克转录因子2(HSF2)检测试剂盒 Rat HSF2 (Heat Shock Transcription Factor 2) ELISA Kit
HZ-EL-R0484c 大鼠音猬因子(SHH) 检测试剂盒 Rat SHH (Hedgehog Homolog, Sonic) ELISA Kit
HZ-EL-R0485c 大鼠Hedgehog相互作用蛋白(HHIP)检测试剂盒 Rat HHIP (Hedgehog Interacting Protein) ELISA Kit
HZ-EL-R0486c 大鼠解旋酶样转录因子(HLTF)检测试剂盒 Rat HLTF (Helicase Like Transcription Factor) ELISA Kit
HZ-EL-R0487c 大鼠幽门螺旋菌IgG(Hp-IgG)检测试剂盒 Rat Hp-IgG (Helicobacter Pylori IgG) ELISA Kit
HZ-EL-R0488c 大鼠血红素氧合酶1(HO-1)检测试剂盒 Rat HO-1 (Heme Oxygenase 1) ELISA Kit
HZ-EL-R0489c 大鼠血红素氧合酶2(HO-2)检测试剂盒 Rat HO-2 (Heme Oxygenase 2) ELISA Kit