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大鼠以红藻氨酸为激动剂的离子型谷氨酸受体2(GRIK2)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠以红藻氨酸为激动剂的离子型谷氨酸受体2(GRIK2)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠以红藻氨酸为激动剂的离子型谷氨酸受体2(GRIK2)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠以红藻氨酸为激动剂的离子型谷氨酸受体2(GRIK2)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠以红藻氨酸为激动剂的离子型谷氨酸受体2(GRIK2)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-P0724c 猪不均一核糖核蛋白A2/B1 (HNRPA2B1)检测试剂盒 Porcine HNRPA2B1 (Heterogeneous Nuclear Ribonucleoprotein A2/B1) ELISA Kit
HZ-EL-P0725c 猪二氢硫辛酸转乙酰酶(DLAT)检测试剂盒 Porcine DLAT (Dihydrolipoyl Transacetylase) ELISA Kit
HZ-EL-P0726c 猪蛋白酪氨酸磷酸酶受体B(PTPRB)检测试剂盒 Porcine PTPRB (Protein Tyrosine Phosphatase Receptor Type B ) ELISA Kit
HZ-EL-P0727c 猪白介素2受体α(IL-2Rα/CD25)检测试剂盒 Porcine IL-2Rα (Interleukin 2 Receptor Alpha) ELISA Kit
HZ-EL-P0728c 猪表面膜免疫球蛋白D(mIgD)检测试剂盒 Porcine MIgD (Membrane IgD) ELISA Kit
HZ-EL-P0729c 猪细胞周期素D1(CCND1)检测试剂盒 Porcine CCND1 (Cyclin-D1) ELISA Kit
HZ-EL-P0730c 猪抗中性粒细胞弹性蛋白酶抗体(ANEA)检测试剂盒 Porcine ANEA (Anti-Neutrophil Elastase Antibody) ELISA Kit
HZ-EL-P0731c 猪胰岛素样生长因子结合蛋白1(IGFBP-1)检测试剂盒 Porcine IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit
HZ-EL-P0732c 猪肝配蛋白A3 (EFNA3)检测试剂盒 Porcine EFNA3 (Ephrin A3) ELISA Kit
HZ-EL-P0734c 猪半胱天冬酶3(CASP3)检测试剂盒 Porcine CASP3 (Caspase 3) ELISA Kit
HZ-EL-P0735c 猪水通道蛋白3(AQP-3)检测试剂盒 Porcine AQP-3 (Aquaporin 3) ELISA Kit
HZ-EL-P0736c 猪II型前胶原羧基端原肽(PIICP)检测试剂盒 Porcine PIICP (Procollagen II C-Terminal ProPeptide) ELISA Kit
HZ-EL-P0737c 猪泛素特异性肽酶8 (USP8)检测试剂盒 Porcine USP8 (Ubiquitin Specific Peptidase 8) ELISA Kit
HZ-EL-P0738c 猪XIII型胶原 (COL13)检测试剂盒 Porcine COL13 (Collagen Type XIII) ELISA Kit
HZ-EL-P0739c 猪肺表面活性物质相关蛋白B(SPB)检测试剂盒 Porcine SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
HZ-EL-P0742c 猪尾型同源盒转录因子(CDX2)检测试剂盒 Porcine CDX2 (Caudal Type Homeobox Transcription Factor 2) ELISA Kit
HZ-EL-P0744c 猪碱性成纤维细胞生长因子(bFGF/FGF2)检测试剂盒 Porcine bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
HZ-EL-P0745c 猪c-jun检测试剂盒 Porcine c-jun ELISA Kit
HZ-EL-P0746c 猪6-羟多巴胺(6-OHDA)检测试剂盒 Porcine 6-OHDA (6-Hydroxydopamine) ELISA Kit
HZ-EL-P0747c 猪CXC趋化因子受体3(CXCR3)检测试剂盒 Porcine CXCR3 (Chemokine CXC Receptor 3) ELISA Kit
HZ-EL-P0748c 猪血小板衍生生长因子D(FDGFD)检测试剂盒 Porcine PDGFD (Platelet Derived Growth Factor D) ELISA Kit
HZ-EL-P0749c 猪细胞外基质金属蛋白酶诱导因子(EMMPRIN/CD147)检测试剂盒 Porcine EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
HZ-EL-P0750c 猪生长调节致癌基因γ(GROγ)检测试剂盒 Porcine GROγ (Growth Regulated Oncogene Gamma) ELISA Kit
HZ-EL-P0751c 猪尿游离皮质醇(UFC)检测试剂盒 Porcine UFC (Urinary Free Cortisol) ELISA Kit
HZ-EL-P0753c 猪磷酯酰肌醇特异性磷脂酶Cβ1(PLCβ1/PI-PLC)检测试剂盒 Porcine PLCβ1/PI-PLC (Phospholipase C Beta 1, Phosphoinositide Specific) ELISA Kit