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大鼠全段甲状旁腺素(I-PTH)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠全段甲状旁腺素(I-PTH)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠全段甲状旁腺素(I-PTH)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠全段甲状旁腺素(I-PTH)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠全段甲状旁腺素(I-PTH)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0419c 猴去唾液酸糖蛋白受体2(ASGR2)检测试剂盒 Monkey ASGR2 (Asialoglycoprotein Receptor 2) ELISA Kit
HZ-EL-MK0420c 猴抑制蛋白β1(ARRβ1)检测试剂盒 Monkey ARRβ1 (Arrestin Beta 1) ELISA Kit
HZ-EL-MK0421c 猴白介素17(IL-17)检测试剂盒 Monkey IL-17 (Interleukin 17) ELISA Kit
HZ-EL-MK0422c 猴胰岛素样生长因子结合蛋白5(IGFBP-5)检测试剂盒 Monkey IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
HZ-EL-MK0423c 猴核转录因子Y亚基γ(NFYC)检测试剂盒 Monkey NFYC (Nuclear Transcription Factor Y Gamma) ELISA Kit
HZ-EL-MK0424c 猴粘蛋白6(MUC6)检测试剂盒 Monkey MUC6 (Mucin 6) ELISA Kit
HZ-EL-MK0425c 猴Toll样受体2(TLR2)检测试剂盒 Monkey TLR2 (Toll-Like Receptor 2) ELISA Kit
HZ-EL-MK0426c 猴泛素羧基端酯酶L1(UCHL1)检测试剂盒 Monkey UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
HZ-EL-MK0427c 猴转化生长因子β刺激蛋白22(TSC22)检测试剂盒 Monkey TSC22 (Transforming Growth Factor Beta Stimulated Protein Clone 22) ELISA Kit
HZ-EL-MK0428c 猴胶原酶II(Collagenase II)检测试剂盒 Monkey Collagenase II ELISA Kit
HZ-EL-MK0429c 猴水通道蛋白3(AQP-3)检测试剂盒 Monkey AQP-3 (Aquaporin 3) ELISA Kit
HZ-EL-MK0430c 猴基质细胞衍生因子4(SDF-4)检测试剂盒 Monkey SDF-4 (Stromal Cell Derived Factor 4) ELISA Kit
HZ-EL-MK0431c 猴肺表面活性物质相关蛋白B(SPB)检测试剂盒 Monkey SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit
HZ-EL-MK0433c 猴膜联蛋白A5(ANXA5 )检测试剂盒 Monkey ANXA5 (Annexin A5) ELISA Kit
HZ-EL-MK0434c 猴细胞因子受体样因子1(CRLF1)检测试剂盒 Monkey CRLF1 (Cytokine Receptor Like Factor 1) ELISA Kit
HZ-EL-MK0435c 猴蛋白酶激活受体1(PAR1)检测试剂盒 Monkey PAR1 (Protease Activated Receptor 1) ELISA Kit
HZ-EL-MK0436c 猴磷脂转运蛋白(PLTP)检测试剂盒 Monkey PLTP (Phospholipid Transfer Protein) ELISA Kit
HZ-EL-MK0437c 猴GATA结合蛋白1(GATA1)检测试剂盒 Monkey GATA1 (GATA Binding Protein 1) ELISA Kit
HZ-EL-MK0438c 猴TU3A蛋白(TU3A)检测试剂盒 Monkey TU3A (TU3A Protein) ELISA Kit
HZ-EL-MK0441c 猴血小板衍生生长因子亚基A(PDGFA)检测试剂盒 Monkey PDGFA (Platelet Derived Growth Factor Subunit A ) ELISA Kit
HZ-EL-MK0442c 猴OJ抗体/抗异亮氨酰tRNA合成酶抗体(OJ/IleRS)检测试剂盒 Monkey OJ/IleRS (Anti-OJ-Antibody) ELISA Kit
HZ-EL-MK0443c 猴III型胶原α1(COL3α1)检测试剂盒 Monkey COL3α1 (Collagen Type III Alpha 1) ELISA Kit
HZ-EL-MK0444c 猴蛋白酶3(PR3)检测试剂盒 Monkey PR3 (Proteinase 3) ELISA Kit
HZ-EL-MK0445c 猴细胞外基质磷酸糖蛋白(MEPE)检测试剂盒 Monkey MEPE (Matrix Extracellular Phosphoglycoprotein) ELISA Kit
HZ-EL-MK0446c 猴半胱天冬酶1(CASP1)检测试剂盒 Monkey CASP1 (Caspase 1) ELISA Kit
HZ-EL-MK0447c 猴纤连蛋白富亮氨酸跨膜蛋白2(FLRT2)检测试剂盒 Monkey FLRT2 (Fibronectin Leucine Rich Transmembrane Protein 2) ELISA Kit