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大鼠主要组织相容性复合体Ⅲ类(MHCⅢ/AgBⅢ/H-1Ⅲ)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠主要组织相容性复合体Ⅲ类(MHCⅢ/AgBⅢ/H-1Ⅲ)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠主要组织相容性复合体Ⅲ类(MHCⅢ/AgBⅢ/H-1Ⅲ)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠主要组织相容性复合体Ⅲ类(MHCⅢ/AgBⅢ/H-1Ⅲ)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠主要组织相容性复合体Ⅲ类(MHCⅢ/AgBⅢ/H-1Ⅲ)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB0274c 兔中间α球蛋白抑制因子H4(ITIH4)检测试剂盒 Rabbit ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit
HZ-EL-RB0277c 兔C-肽(C-P)检测试剂盒 Rabbit C-P (C-Peptide) ELISA Kit
HZ-EL-RB0278c 兔补体因子D(CFD)检测试剂盒 Rabbit CFD (Complement Factor D) ELISA Kit
HZ-EL-RB0279c 兔普列克底物蛋白同源物样结构域家族A成员2(PHLDA2)检测试剂盒 Rabbit PHLDA2 (Pleckstrin Homology Like Domain Family A, Member 2) ELISA Kit
HZ-EL-RB0280c 兔微管蛋白α3C(TUBα3C)检测试剂盒 Rabbit TUBα3C (Tubulin Alpha 3c )ELISA Kit
HZ-EL-RB0282c 兔γ1肌动蛋白(ACTG1)检测试剂盒 Rabbit ACTg1 (Actin Gamma 1) ELISA Kit
HZ-EL-RB0283c 兔肿瘤特异生长因子/肿瘤相关因子(TSGF)检测试剂盒 Rabbit TSGF (Tumor Specific Growth Facter/Tumor Supplied Group of Factor) ELISA Kit
HZ-EL-RB0284c 兔过氧化还原酶5(PRDX5)检测试剂盒 Rabbit PRDX5 (Peroxiredoxin 5) ELISA Kit
HZ-EL-RB0285c 兔半胱氨酸蛋白酶抑制剂(CSTA)检测试剂盒 Rabbit CSTA (Cystatin A) ELISA Kit
HZ-EL-RB0286c 兔嗜环蛋白/亲环素D(CYPD)检测试剂盒 Rabbit CYPD (Cyclophilin D) ELISA Kit
HZ-EL-RB0287c 兔抗磷脂酰丝氨酸抗体(APSA)检测试剂盒 Rabbit APSA (Anti-Phosphatidyl Serine Antibody) ELISA Kit
HZ-EL-RB0288c 兔髓系细胞触发受体-1(TREM-1)检测试剂盒 Rabbit TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
HZ-EL-RB0289c 兔性别决定区Y框蛋白2(SOX2)检测试剂盒 Rabbit SOX2 (Sex Determining Region Y Box Protein 2) ELISA Kit
HZ-EL-RB0290c 兔B细胞连接蛋白(BLNK)检测试剂盒 Rabbit BLNK (B-Cell Linker Protein) ELISA Kit
HZ-EL-RB0291c 兔鸟苷酸激酶1(GUK1)检测试剂盒 Rabbit GUK1 (Guanylate Kinase 1) ELISA Kit
HZ-EL-RB0292c 兔脂联素受体1(ADIPOR1)检测试剂盒 Rabbit ADIPOR1 (Adiponectin Receptor 1) ELISA Kit
HZ-EL-RB0293c 兔促肾上腺皮质激素释放激素结合蛋白(CRHBP)检测试剂盒 Rabbit CRHBP (Corticotropin Releasing Hormone Bingding Protein) ELISA Kit
HZ-EL-RB0294c 兔细胞周期素D2(CCND2)检测试剂盒 Rabbit CCND2 (Cyclin-D2) ELISA Kit
HZ-EL-RB0295c 兔丝裂原激活蛋白激酶激酶1(MAP2K1)检测试剂盒 Rabbit MAP2K1 (Mitogen Activated Protein Kinase Kinase 1) ELISA Kit
HZ-EL-RB0296c 兔凝血因子Ⅻ(F12)检测试剂盒 Rabbit F12 (Coagulation Factor Ⅻ) ELISA Kit
HZ-EL-RB0297c 兔谷氨酸脱羧酶(GAD)检测试剂盒 Rabbit GAD (Glutamic Acid Decarboxylase) ELISA Kit
HZ-EL-RB0298c 兔趋化因子CC受体8(CCR8)检测试剂盒 Rabbit CCR8 (Chemokine C-C-Motif Receptor 8) ELISA Kit
HZ-EL-RB0299c 兔烟酰胺腺嘌呤二核苷酸磷酸(NADPH)检测试剂盒 Rabbit NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit
HZ-EL-RB0301c 兔凝血酶受体(TR)检测试剂盒 Rabbit TR (Thrombin Receptor) ELISA Kit