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大鼠幽门螺旋菌IgM(Hp-IgM)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠幽门螺旋菌IgM(Hp-IgM)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠幽门螺旋菌IgM(Hp-IgM)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠幽门螺旋菌IgM(Hp-IgM)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠幽门螺旋菌IgM(Hp-IgM)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB1174c 兔白介素23 p40 (IL-23 p40)检测试剂盒 Rabbit IL-23 p40(Interleukin 23) ELISA Kit
HZ-EL-RB1175c 兔蛋白C(PROC)检测试剂盒 Rabbit PROC (Protein C) ELISA Kit
HZ-EL-RB1177c 兔胰腺再生蛋白1β(REG1β)检测试剂盒 Rabbit REG1β (Regenerating Islet Derived Protein 1 Beta) ELISA Kit
HZ-EL-RB1178c 兔心肌营养素样细胞因子1(CLCF1)检测试剂盒 Rabbit CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit
HZ-EL-RB1179c 兔成熟促进因子(MPF)检测试剂盒 Rabbit MPF (Maturation Promoting Factor) ELISA Kit
HZ-EL-RB1180c 兔肌细胞生成素(MYOG)检测试剂盒 Rabbit MYOG (Myogenin) ELISA Kit
HZ-EL-RB1181c 兔细胞分裂周期蛋白23(CDC23)检测试剂盒 Rabbit CDC23 (Cell Division Cycle Protein 23) ELISA Kit
HZ-EL-RB1182c 兔血小板相关补体3(PAC3)检测试剂盒 Rabbit PAC3 (Platelet Associated Complement 3) ELISA Kit
HZ-EL-RB1183c 兔核孔蛋白155kda(NUP155)检测试剂盒 Rabbit NUP155 (Nucleoporin 155kDa) ELISA Kit
HZ-EL-RB1184c 兔重酒石酸去甲肾上腺素(NEB)检测试剂盒 Rabbit NEB (Noradrenaline Bitartas) ELISA Kit
HZ-EL-RB1185c 兔糖化白蛋白(GA)检测试剂盒 Rabbit GA (Glycated Albumin) ELISA Kit
HZ-EL-RB1187c 兔嗜铬蛋白A/胰抑制素(CgA/Pancreastatin)检测试剂盒 Rabbit CgA (Chromogranin A/Pancreastatin) ELISA Kit
HZ-EL-RB1188c 兔降钙素受体(CTR)检测试剂盒 Rabbit CTR (Calcitonin Receptor) ELISA Kit
HZ-EL-RB1190c 兔β2糖蛋白1抗体IgM(β2-GP1 Ab IgM)检测试剂盒 Rabbit β2-GP1 Ab IgM (β2-Glycoprotein 1 Antibody IgM) ELISA Kit
HZ-EL-RB1191c 兔硒蛋白1(SEP1)检测试剂盒 Rabbit SEP1 (Selenoprotein 1) ELISA Kit
HZ-EL-RB1193c 兔肌肉糖原磷酸化酶(PYGM)检测试剂盒 Rabbit PYGM (Glycogen Phosphorylase, Muscle) ELISA Kit
HZ-EL-RB1194c 兔甲胎蛋白异质体3(AFP-L3)检测试剂盒 Rabbit AFP-L3 (Alpha-Fetoprotein Lens Culinaris Agglutinin 3) ELISA Kit
HZ-EL-RB1195c 兔血幼素(HJV/HFE2)检测试剂盒 Rabbit HJV/HFE2 (Hemojuvelin/Hemochromatosis Type 2) ELISA Kit
HZ-EL-RB1196c 兔αL艾杜糖醛酸酶(IDUα)检测试剂盒 Rabbit IDUα (Alpha-L-Iduronidase) ELISA Kit
HZ-EL-RB1197c 兔Ras-GTP酶激活蛋白1(Ras-GAP1)检测试剂盒 Rabbit Ras-GAP1 (Rac-GTPase Activating Protein 1) ELISA Kit
HZ-EL-RB1198c 兔干细胞因子(SCF)检测试剂盒 Rabbit SCF (Stem Cell Factor) ELISA Kit
HZ-EL-RB1199c 兔S100钙结合蛋白A11(S100A11)检测试剂盒 Rabbit S100A11 (S100 Calcium Binding Protein A11) ELISA Kit
HZ-EL-RB1200c 兔Bcl-2样蛋白1(BCL2L1/BCL-X)检测试剂盒 Rabbit BCL2L1/BCL-X (Bcl-2 Like Protein 1) ELISA Kit
HZ-EL-RB1201c 兔甘露糖受体C1(MRC1)检测试剂盒 Rabbit MRC1 (Mannose Receptor C Type 1 ) ELISA Kit
HZ-EL-RB1202c 兔凝血酶(TM)检测试剂盒 Rabbit TM (Thrombin)ELISA Kit
HZ-EL-RB1203c 兔膜联蛋白A2(ANXA2 )检测试剂盒 Rabbit ANXA2 (Annexin A2) ELISA Kit
HZ-EL-RB1204c 兔激酶锚定蛋白1(AKAP1)检测试剂盒 Rabbit AKAP1 (A Kinase Anchor Protein 1) ELISA Kit
HZ-EL-RB1205c 兔血栓前体蛋白(TpP)检测试剂盒 Rabbit TPP (Thrombus Precursor Protein) ELISA Kit