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大鼠丙酮酸脱氢酶α(PDHA)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠丙酮酸脱氢酶α(PDHA)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠丙酮酸脱氢酶α(PDHA)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠丙酮酸脱氢酶α(PDHA)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠丙酮酸脱氢酶α(PDHA)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-P0424c 猪蛋白激酶Bγ(PKBG)检测试剂盒 Porcine PKBG (Protein Kinase B Gamma) ELISA Kit
HZ-EL-P0425c 猪主要穹窿蛋白(MVP)检测试剂盒 Porcine MVP (Major Vault Protein) ELISA Kit
HZ-EL-P0426c 猪血管内皮生长因子受体2(VEGFR-2/KDR)检测试剂盒 Porcine VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
HZ-EL-P0427c 猪载脂蛋白B(ApoB)检测试剂盒 Porcine ApoB (Apolipoprotein B) ELISA Kit
HZ-EL-P0428c 猪雌激素受体α(ERα)检测试剂盒 Porcine ERα (Estrogen Receptor Alpha) ELISA Kit
HZ-EL-P0429c 猪核糖核酸酶H(RNASEH)检测试剂盒 Porcine RNASEH (Ribonuclease H ) ELISA Kit
HZ-EL-P0430c 猪可溶性糖蛋白130(sgp130)检测试剂盒 Porcine sgp130 (soluble Glucoprotein 130) ELISA Kit
HZ-EL-P0431c 猪抑瘤素M(OSM)检测试剂盒 Porcine OSM (Oncostatin M) ELISA Kit
HZ-EL-P0432c 猪基膜聚糖(LUM)检测试剂盒 Porcine LUM (Lumican) ELISA Kit
HZ-EL-P0433c 猪微球粒蛋白1(MCRS1)检测试剂盒 Porcine MCRS1 (Microspherule Protein 1) ELISA Kit
HZ-EL-P0434c 猪尿嘧啶核苷磷酸化酶2(UPP2)检测试剂盒 Porcine UPP2 (Uridine Phosphorylase 2) ELISA Kit
HZ-EL-P0435c 猪信号素3B (SEMA3B)检测试剂盒 Porcine SEMA3B (Semaphorin 3B) ELISA Kit
HZ-EL-P0436c 猪铁蛋白(FE)检测试剂盒 Porcine FE (Ferritin) ELISA Kit
HZ-EL-P0437c 猪抗小核糖核蛋白/Sm抗体(snRNP/Sm)检测试剂盒 Porcine snRNP/Sm (Small Nuclear Ribonucleoprotein) ELISA Kit
HZ-EL-P0438c 猪钙联蛋白(CNX)检测试剂盒 Porcine CNX (Calnexin) ELISA Kit
HZ-EL-P0439c 猪抑制蛋白β1(ARRβ1)检测试剂盒 Porcine ARRβ1 (Arrestin Beta 1) ELISA Kit
HZ-EL-P0440c 猪半乳凝集素4(GAL4)检测试剂盒 Porcine GAL4 (Galectin 4) ELISA Kit
HZ-EL-P0441c 猪精氨酸加压素受体1A(AVPR1A)检测试剂盒 Porcine AVPR1A (Arginine Vasopressin Receptor 1A) ELISA Kit
HZ-EL-P0442c 猪BH3结构域凋亡诱导蛋白(Bid)检测试剂盒 Porcine Bid (BH3 Interacting Domain Death Agonist) ELISA Kit
HZ-EL-P0443c 猪补体片段3c(C3c)检测试剂盒 Porcine C3c (Complement Fragment 3c) ELISA Kit
HZ-EL-P0444c 猪胸腺非依赖性抗原(TI-Ag)检测试剂盒 Porcine TI-Ag (Thymus Independent Antigen) ELISA Kit
HZ-EL-P0446c 猪磷酸化核因子κB抑制蛋白α(pIκBα)检测试剂盒 Porcine pIκBα (Phospho-Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
HZ-EL-P0447c 猪细胞外信号调节激酶(ERK)检测试剂盒 Porcine ERK (Extracellular Signal Regulated Kinase) ELISA Kit
HZ-EL-P0448c 猪妊娠相关血浆蛋白2(PAPPA2)检测试剂盒 Porcine PAPPA2 (Pappalysin 2) ELISA Kit
HZ-EL-P0450c 猪蛋白磷酸酶(PP)检测试剂盒 Porcine PP (Protein Phosphatase) ELISA Kit
HZ-EL-P0451c 猪胆固醇酯转移蛋白(CETP)检测试剂盒 Porcine CETP (Cholesteryl Ester Transfer Protein) ELISA Kit
HZ-EL-P0452c 猪速激肽受体3(TACR3)检测试剂盒 Porcine TACR3 (Tachykinin Receptor 3) ELISA Kit