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大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠呼吸道合胞病毒IgM(RSV- IgM)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-P0913c 猪信号传导转录激活因子6B(STAT56B)检测试剂盒 Porcine STAT6B (Signal Transducer and Activator of Transcription 6B) ELISA Kit
HZ-EL-P0914c 猪血浆五聚蛋白3 (PTX 3/TSG-14)检测试剂盒 Porcine PTX 3/TSG-14 (Pentraxin 3, Long) ELISA Kit
HZ-EL-P0915c 猪血小板型磷酸果糖激酶(PFKP)检测试剂盒 Porcine PFKP (Phosphofructokinase, Platelet) ELISA Kit
HZ-EL-P0916c 猪糖原合成酶激酶3α(GSK3α)检测试剂盒 Porcine GSK3α (Glycogen Synthase Kinase 3 Alpha) ELISA Kit
HZ-EL-P0917c 猪甲状腺素抗体(TAb)检测试剂盒 Porcine TAB (Thyroxine Antibody) ELISA Kit
HZ-EL-P0918c 猪芳香酶(ARO)检测试剂盒 Porcine ARO (Aromatase) ELISA Kit
HZ-EL-P0919c 猪降钙素(CT)检测试剂盒 Porcine CT (Calcitonin) ELISA Kit
HZ-EL-P0920c 猪尿嘧啶核苷磷酸化酶1(UPP1)检测试剂盒 Porcine UPP1 (Uridine Phosphorylase 1) ELISA Kit
HZ-EL-P0921c 猪生长因子受体结合蛋白14(Grb14)检测试剂盒 Porcine Grb14 (Growth Factor Receptor Bound Protein 14) ELISA Kit
HZ-EL-P0922c 猪丙酮酸脱氢酶β(PDHβ)检测试剂盒 Porcine PDHβ (Pyruvate Dehydrogenase Beta) ELISA Kit
HZ-EL-P0923c 猪衰老关键蛋白4(FBLN4)检测试剂盒 Porcine FBLN4 (Fibulin 4) ELISA Kit
HZ-EL-P0924c 猪脂质运载蛋白1(LCN1)检测试剂盒 Porcine LCN1 (Lipocalin 1) ELISA Kit
HZ-EL-P0925c 猪核基质蛋白22(NMP-22)检测试剂盒 Porcine NMP-22 (Nuclear Matrix Protein 22) ELISA Kit
HZ-EL-P0926c 猪晶状体蛋白αB (CRYαB)检测试剂盒 Porcine CRYαB (Crystallin Alpha B) ELISA Kit
HZ-EL-P0927c 猪胱天蛋白酶14(CASP14)检测试剂盒 Porcine CASP14 (Caspase 14) ELISA Kit
HZ-EL-P0928c 猪松弛素/胰岛素样肽受体1(RXFP1)检测试剂盒 Porcine RXFP1 (Relaxin/Insulin Like Family Peptide Receptor 1) ELISA Kit
HZ-EL-P0931c 猪血小板膜糖蛋白II b/III a(GPIIb/IIIa)检测试剂盒 Porcine GPIIb/IIIa (Platelet Membrane Glycoprotein IIb/IIIa) ELISA Kit
HZ-EL-P0932c 猪多聚免疫球蛋白受体(PIGR/SC)检测试剂盒 Porcine PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) ELISA Kit
HZ-EL-P0934c 猪α1连接素(CTNNα1)检测试剂盒 Porcine CTNNα1 (Catenin, Alpha 1) ELISA Kit
HZ-EL-P0935c 猪嗜酸粒细胞趋化因子受体(ECFR)检测试剂盒 Porcine ECFR (Eosinophil Chemotactic Factor Receptor) ELISA Kit
HZ-EL-P0936c 猪粘膜相关上皮趋化因子(MEC/CCL28)检测试剂盒 Porcine MEC/CCL28 (Mucosae Associated Epithelia Chemokine) ELISA Kit
HZ-EL-P0938c 猪白血病抑制因子受体(LIFR)检测试剂盒 Porcine LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit
HZ-EL-P0940c 猪晶体蛋白(CLC)检测试剂盒 Porcine CLC (Charcot Leyden Crystal Protein) ELISA Kit
HZ-EL-P0941c 猪髓鞘相关糖蛋白(MAG)检测试剂盒 Porcine MAG (Myelin Associated Glycoprotein) ELISA Kit
HZ-EL-P0942c 猪11去氢血栓烷B2(11-DH-TXB2)检测试剂盒 Porcine 11-DH-TXB2 (11-Dehydro-thromboxane B2) ELISA Kit
HZ-EL-P0943c 猪抗角蛋白丝聚集素/丝集蛋白抗体(AFA)检测试剂盒 Porcine AFA (Anti-Filaggrin Antibody) ELISA Kit
HZ-EL-P0947c 猪FMS样酪氨酸激酶3(Flt3)检测试剂盒 Porcine Flt3 (FMS Like Tyrosine Kinase 3) ELISA Kit
HZ-EL-P0948c 猪前胶原C端肽酶(PCP)检测试剂盒 Porcine PCP (Procollagen C Peptidase) ELISA Kit