产品详情
Product Name: Streptavidin and Conjugates
别名: | 抗生物素蛋白链菌素,链亲合素;Streptavidin 来源于阿维丁链霉菌 |
CAS号: | 9013-20-1 |
MDL号: | MFCD00082035 |
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介绍: | Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. |
用途: | Applications for enzyme-labeled (HRP, AP) streptavidin: ?Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies ?Cell and tissue staining (for light microscopy) ?Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: ?Immunofluorescent assays (IFA) ?Tissue staining (for fluorescence microscopy) ?Blot immunostaining ?Cell staining (incl. fluorescent activated cell sorting) |
贮存: | ?20°C 保存 |
生化和生理学机理: | reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation. |
备注: | 单位定义:1单位结链霉亲合素合1.0 UG 生物素. 方法描述见文献 GREEN N.M., METHODS IN ENZYMOLOGY, 18, 418 (1970). 来源:Streptomyces avidinii |
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A0002D-丙氨酸/D-2-氨基丙酸/D-氨基丙酸/D-初油氨基酸/D-丝析氨酸/ D-丙胺酸/D-α-氨基丙酸/D-α-丙氨酸/D-AlanineBR,99%
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A0011DL-精氨酸/DL-蛋白氨基酸/DL-胍基戊氨酸/DL-2-氨基-5-胍基戊酸/DL-ArginineBR,98%
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A0022L-天冬氨酸/L-天门冬氨酸/L-氨基丁二酸/L-氨基琥珀酸/L-天冬酸/L-Aspartic acidBR,99%
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