英文名称    Phospho-SHIP1 (Tyr1020)    

中文名称    磷酸化SH2结构含磷酸肌醇SHIP1抗体    

别    名    SHIP1 (Phospho Tyr1020); SHIP1 (Phospho-Tyr1020); SHIP1 (Phospho Y1020); P-SHIP1 (Tyr1020); Inositol polyphosphate 5 phosphatase of 145kDa; 4; 5-trisphosphate 5-phosphatase 1; hp51CN; hSHIP; Inositol polyphosphate 5 phosphatase 145kDa; Inositol polyphosphate 5 phosphatase; Inositol polyphosphate 5 phosphatase D; Inositol polyphosphate-5-phosphatase of 145 kDa; INPP 5D; INPP5D; INPP5D protein; MGC104855; MGC142140; MGC142142; p150 ship; p150Ship; Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1; Phosphatidylinositol-3; SH2 containing inositol 5 phosphatase; SH2 containing inositol phosphatase isoform b; SH2 domain containing inositol 5' phosphatase 1; SH2 domain containing inositol phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5''-phosphatase 1; SHIP 1; SHIP-1; SHIP1; SHIP1_RAT; Signaling inositol polyphosphate 5 phosphatase SIP 145; Signaling inositol polyphosphate 5 phosphatase SIP145; SIP 145; SIP-145; SIP145.    

磷酸化SH2结构含磷酸肌醇SHIP1抗体        

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    免疫学  信号转导  激酶和磷酸酶  t-淋巴细胞      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    134kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from rat SHIP1 around the phosphorylation site of Tyr1020    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化SH2结构含磷酸肌醇SHIP1抗体产品介绍    background:
SHIP1 is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and contains an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation in response to multiple cytokine and B and T cell receptor activation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall the protein functions as a negative regulator of myeliod cell proliferation and survival.

Function:
Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6 (By similarity).

Subunit:
Interacts with tyrosine phosphorylated forms of SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150. Interacts with PTPN11 in response to IL-3. Interacts with receptors EPOR, MS4A2/FCER1B and FCER1G, FCGR2A, FCGR2B and FCGR3. Interacts with GRB2 and PLCG1. Interacts with tyrosine kinases SRC and TEC. Interacts with FCGR2A, leading to regulate gene expression during the phagocytic process. Interacts with c-Met/MET (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Can weakly interact (via NPXY motif 2) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif.

Subcellular Location:
Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). Note=Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate in membrane localization (By similarity).

Tissue Specificity:
Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells.

Post-translational modifications:
Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

Similarity:
Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. 
Contains 1 SH2 domain.

Gene ID:
65038

Database links:

Entrez Gene: 3635 Human

Entrez Gene: 16331 Mouse

Omim: 601582 Human

SwissProt: Q92835 Human

SwissProt: Q9ES52 Mouse

Unigene: 262886 Human

Unigene: 601911 Human

Unigene: 15105 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化SH2结构含磷酸肌醇SHIP1抗体

hz-3452R Phospho-Rb (Thr821)  磷酸化视网膜母细胞瘤相关蛋白1抗体

hz-5653R Phospho-Rb(Ser612)  磷酸化视网膜母细胞瘤相关蛋白1抗体

hz-5665R Phospho-Rb(Ser788)  磷酸化视网膜母细胞瘤相关蛋白1抗体

hz-5666R Phospho-Rb(Ser795)  磷酸化视网膜母细胞瘤相关蛋白1抗体

hz-5667R Phospho-Rb(Thr826)  磷酸化视网膜母细胞瘤相关蛋白1抗体

hz-0645R Rat secretory IgA  分泌型免疫球蛋白A抗体

hz-2901R RECK  金属蛋白酶抑制因子RECK抗体

hz-3691R REG4/RELP  再生基因蛋白4抗体

hz-1560R RELN/Reelin  络丝蛋白抗体

hz-1883R Rbms3  抑癌基因Rbms3抗体

hz-2793R RET/C ret  指状蛋白RET抗体

hz-3384R Phospho-Ret (Tyr905)  磷酸化指状蛋白RET抗体

hz-3385R Phospho-Ret (Tyr1062)  磷酸化指状蛋白RET抗体

hz-0795R Resistin  抵抗素抗体

hz-2214R Resistin  抵抗素抗体

hz-1884R RELMa/RELM alpha  抵抗素样分子α抗体

hz-3562R RelB  核转录因子NFKB-RelB蛋白抗体

hz-3383R Phospho-RelB(Ser552)  磷酸化核转录因子NFKB-RelB蛋白抗体

hz-5692R Phospho-RelB(Ser573)  磷酸化核转录因子NFKB-RelB蛋白抗体

hz-1885R Reprimo  细胞质内的糖基化蛋白抗体

hz-2666R C-REL  淋巴细胞衍生C-型凝集素抗体

hz-0492R RGS2  G蛋白信号转导调节因子2抗体

hz-2794R RGS5  G蛋白信号转导调节因子5抗体

hz-3867R RGS19  G蛋白信号转导调节因子19抗体

hz-5686R phospho-RGS19(Ser22)  磷酸化G蛋白信号转导调节因子19抗体

hz-5687R phospho-RGS19(Ser24)  磷酸化G蛋白信号转导调节因子19抗体

hz-5688R phospho-RGS19(Ser151)  磷酸化G蛋白信号转导调节因子19抗体

hz-5689R phospho-RGS19(Tyr143)  磷酸化G蛋白信号转导调节因子19抗体

hz-1180R RhoA  RhoA抗体

hz-1480R Rho C  RhoC抗体

hz-5949R RNF56/CBLB  环指蛋白56

hz-3546R RIP2  受体结合丝氨酸苏氨酸激酶2抗体

hz-3361R Phospho-RIP2 (Ser176)  磷酸化受体结合丝氨酸苏氨酸激酶2抗体

hz-3551R RIPK3  受体结合丝氨酸苏氨酸激酶3抗体

hz-3564R RICTOR  雷帕霉素靶蛋白复合体2抗体

hz-3387R Phospho-Rictor (Thr1135)  磷酸化雷帕霉素靶蛋白复合体2抗体

hz-2125R RNF126  环指蛋白126抗体